We narrowed to 6,452 results for: itch

1. pET28:LanII-2C

   Plasmid

   #225070

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2C)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28:LanII-2A

   Plasmid

   #225068

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28:LanII-2B

   Plasmid

   #225069

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2B)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28:LanIII-43

   Plasmid

   #225077

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIII-43)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28:LanII-57

   Plasmid

   #225074

   Purpose

   Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA(A) (LanII-57)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28:LanIII-6

   Plasmid

   #225076

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA(A) (LanIII-6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28:LanII-2E

   Plasmid

   #225072

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2E)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28:LanII-23

   Plasmid

   #225067

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-23)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28:LanII

   Plasmid

   #225066

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28:LanII-2D

    Plasmid

    #225071

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2D)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-PODXL S481D

    Plasmid

    #195224

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S481D

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCD510B attp BSD

    Plasmid

    #216236

    Purpose

    Lentiviral vector, For genome integration of an attP PhiC13 recognition site

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    blasticidin S deaminase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pILGFPEasy9R

    Plasmid

    #218291

    Purpose

    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRL-VinculinTS-S1033A

    Plasmid

    #211835

    Purpose

    Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinTS-S1033A (VCL Synthetic, Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mutated vinculin serine 1033 to alanine (S1033A),…

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL-VinculinTS-S1033D

    Plasmid

    #211893

    Purpose

    Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinTS-S1033D (VCL Synthetic, Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mutated vinculin serine 1033 to aspartic acid (S1…

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRRL-VinculinCS-S1033A

    Plasmid

    #211894

    Purpose

    Vinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinCS-S1033A (VCL Synthetic, Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mutated vinculin serine 1033 to alanine (S1033A),…

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRRL-VinculinCS-S1033D

    Plasmid

    #211895

    Purpose

    Vinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinCS-S1033D (VCL Synthetic, Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mutated vinculin serine 1033 to aspartic acid (S1…

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV.5’eGFP/sgLMNA

    Plasmid

    #170546

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of LMNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV.5’eGFP/sgVIM

    Plasmid

    #170548

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of VIM
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p202-Syt1-C2A-wt

    Plasmid

    #201553

    Purpose

    Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Synaptotagmin-1 C2A domain (partial)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli

    Promoter

    RBS

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-Halo-H2DBD

    Plasmid

    #200206

    Purpose

    Expression of truncated HSF2 (DBD)

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HA-Halo-H2DBD (Hsf2 Mouse)
    

    Use

    Tags

    Halo; HA

    Expression

    Mammalian

    Mutation

    HSF2 (1-366; 15 bp linker; 972-1017)

    Promoter

    EF_1alpha

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NLS-Halo-HSF2

    Plasmid

    #200198

    Purpose

    Expression of full length HSF2 with SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Halo-H2DBD-HR

    Plasmid

    #200199

    Purpose

    Expression of truncated HSF2 (DBD and HR)

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-H2DBD-HR (Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    HSF2 (1-627)

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Halo-H2DBD

    Plasmid

    #200200

    Purpose

    Expression of truncated HSF2 (DBD)

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-H2DBD (Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    HSF2 (1-366; 15 bp linker; 972-1017)

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HA-Halo-HSF2

    Plasmid

    #200202

    Purpose

    Expression of full length HSF2

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HA-Halo-HSF2 (Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. H1DBD-Halo-HA

    Plasmid

    #200203

    Purpose

    Expression of truncated HSF1 (DBD and HR)

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    H1DBD-Halo-HA (Hsf1 Mouse)
    

    Use

    Tags

    Halo; HA

    Expression

    Mammalian

    Mutation

    HSF1 (1-399)

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. H1DBD-HR-Halo

    Plasmid

    #200204

    Purpose

    Expression of truncated HSF1 (DBD and HR)

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    H1DBD-HR-Halo (Hsf1 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    HSF1 (1-672)

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Halo-HSF2

    Plasmid

    #200192

    Purpose

    Expression of full length HSF2

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-HSF2 (Hsf2 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ShadowY-CLC

    Plasmid

    #193560

    Purpose

    Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    ShadowY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CLC-ShadowY

    Plasmid

    #193561

    Purpose

    Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    ShadowY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. E42_gRunx3_gRNA3_dTet_mTurquoise2

    Plasmid

    #189806

    Purpose

    Retroviral delivery of guide RNA against mouse Runx3

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx3_gRNA3 (Runx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. E42_gRunx3_gRNA2_dTet_mTurquoise2

    Plasmid

    #189805

    Purpose

    Retroviral delivery of guide RNA against mouse Runx3

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx3_gRNA2 (Runx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. E42_gRunx3_gRNA1_dTet_mTurquoise2

    Plasmid

    #189804

    Purpose

    Retroviral delivery of guide RNA against mouse Runx3

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx3_gRNA1 (Runx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-EGFP-DmEDC3_441-680_D

    Plasmid

    #146127

    Purpose

    Insect Expression of DmEDC3_441-680

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3_441-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    3 mutations N162T, K221E and D418G compared to th…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1B-lambdaN-HA-DmEDC3_441-680_D

    Plasmid

    #146130

    Purpose

    Insect Expression of DmEDC3_441-680

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3_441-680 (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-lambdaN-HA-DmEDC3-D61A_D

    Plasmid

    #146131

    Purpose

    Insect Expression of DmEDC3-D61A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3-D61A (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAc5.1B-lambdaN-HA-DmEDC3-D61K_D

    Plasmid

    #146132

    Purpose

    Insect Expression of DmEDC3-D61K

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3-D61K (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1B-lambdaN-HA-DmEDC3-N44A_D

    Plasmid

    #146135

    Purpose

    Insect Expression of DmEDC3-N44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmEDC3-N44A (Edc3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A456G and three mutations N16…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EcRfaHKOW-L1L2NusG

    Plasmid

    #113126

    Purpose

    Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107, replaced L1 and L2 loops …

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-4T-3-cytXlATL2

    Plasmid

    #124063

    Purpose

    Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXIATL2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge