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pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

Plasmid

#162377

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Has service

AAV1, AAV9, and AAV Retrograde

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Publication

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Promoter

hSyn

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Publication

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pRK-ATF4

Plasmid

#26114

Depositor

Yihong Ye

Publication

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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MSCVhygro-F-Ezh2

Plasmid

#24926

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

June 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Publication

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEGFP(N3)-PP1beta

Plasmid

#44223

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PP1B (PPP1CB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Publication

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pGTR

Plasmid

#63143

Purpose

PCR template to construct polycistronic tRNA-gRNA

Depositor

Yinong Yang

Publication

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA-tRNA fusion

Use

CRISPR and Synthetic Biology

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Stat3 pcDNA3

Plasmid

#8706

Purpose

See Depositor Comments below for variant information.

Depositor

Jim Darnell

Publication

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Stat3 (Stat3 Mouse)

Expression

Mammalian

Mutation

* see comments

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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RCIscript-GoldyTALEN

Plasmid

#38142

Purpose

destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

Depositor

Daniel Carlson, Stephen Ekker

Publication

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Mrna transcription vector

Tags

AcV5 and FokI homodimer

Mutation

Truncate at N and C terminous

Promoter

T3

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Publication

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

Plasmid

#162380

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Has service

AAV1, AAV9, and AAV Retrograde

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CAG

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Publication

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has service

AAV1, AAV5, and AAV Retrograde

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

Plasmid

#130998

Purpose

Double floxed ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Publication

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has service

AAV1 and AAV5

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

Ef1a

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA3-FLAG-KD LKB1

Plasmid

#8591

Depositor

Lewis Cantley

Publication

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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EGFP-Rab7A T22N

Plasmid

#28048

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

T22N

Available since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pGEN-luxCDABE

Plasmid

#44918

Depositor

Harry Mobley

Publication

Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sigma 70 promoter

Use

Luciferase

Tags

lux transcriptional fusion

Promoter

em7

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV1 and AAV Retrograde

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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