We narrowed to 1,587 results for: Tef

1. pCDNA4/TO-SPOP-myc (R121Q)

   Plasmid

   #197007

   Purpose

   Mammalian inducible expression vector for myc-tagged SPOP

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R121Q

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4/TO-SPOP-myc (R354H)

   Plasmid

   #197006

   Purpose

   Mammalian inducible expression vector for myc-tagged SPOP

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R354H

   Promoter

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4/TO-SPOP-myc (R221C)

   Plasmid

   #197005

   Purpose

   Mammalian inducible expression vector for myc-tagged SPOP

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R221C

   Promoter

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4/TO-SPOP-myc (BACK)

   Plasmid

   #197021

   Purpose

   Mammalian inducible expression vector for myc-tagged SPOP

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Y353E

   Promoter

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPEE42

   Plasmid

   #139421

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   Citrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPEE45

   Plasmid

   #139424

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTurquoise1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPEE44

   Plasmid

   #139423

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPEE43

   Plasmid

   #139422

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mMaroon1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWKS8

   Plasmid

   #139415

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTFP1 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWKS17

    Plasmid

    #139420

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_SNORD2 probe

    Plasmid

    #73070

    Purpose

    antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD2 (SNORD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1_SNORD60 probe

    Plasmid

    #73071

    Purpose

    antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD60 (SNORD60 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

    Plasmid

    #115432

    Purpose

    Vector for expressing RPL28 with mutagenized exon2

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    exon 2 deleted, with MfeI / NheI cloning site

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SPOP-V5 (BTB+BACK)

    Plasmid

    #169723

    Purpose

    Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K, Y353E

    Promoter

    CMV

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-NUP188

    Plasmid

    #159893

    Purpose

    Expresses NUP188 in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

    Insert

    NUP188 (NUP188 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    To avoid NotI site, first three amino acids are d…

    Promoter

    CMV

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQFlag-USP7 WT puroR

    Plasmid

    #46751

    Purpose

    Retroviral vector that expresses wild type Flag-tagged human USP7

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNTI647 dCas9-Mxi1 TetR KanMX

    Plasmid

    #139474

    Purpose

    Integrates CRISPRi effector and tet-responsive regulator

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Inserts

    dCas9-Mxi1
    Tet Repressor
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1 and pTEF1

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

    Plasmid

    #216125

    Purpose

    Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge