We narrowed to 5,965 results for: crispr cas9 expression plasmids

1. pEG302 22aa SunTag VP64 nog

   Plasmid

   #120251

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEG302 22aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #120249

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

   Plasmid

   #115486

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEG302 22aa SunTag VP64 g4+g17

   Plasmid

   #120252

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEG302 5aa SunTag VP64 nog

   Plasmid

   #115480

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEG302 22aa SunTag NtDRMcd SUP

   Plasmid

   #115489

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 5aa SunTag VP64 CLV3

   Plasmid

   #115485

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEG302 5aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #115481

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 5aa SunTag VP64 AP3

   Plasmid

   #115484

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEG302 5aa SunTag VP64 gRNA17 (FWA)

    Plasmid

    #115482

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid

    #120250

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX852

    Plasmid

    #62884

    Purpose

    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX854

    Plasmid

    #62886

    Purpose

    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa573-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX853

    Plasmid

    #62885

    Purpose

    N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-573)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Promoter

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJAT58

    Plasmid

    #204304

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJAT71

    Plasmid

    #204305

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge