We narrowed to 5,650 results for: pCas

1. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

   Plasmid

   #197423

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

   Plasmid

   #197424

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

   Plasmid

   #197425

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

   Plasmid

   #197426

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas-Cas12m-ΔZF

   Plasmid

   #192276

   Purpose

   Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   Cas12m ΔZF
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   H459A, C552A

   Promoter

   PJ23108

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas-dCas12m-ΔZF

   Plasmid

   #192277

   Purpose

   pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m ΔZF
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D485A, H549A, C552A

   Promoter

   PJ23108

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-DIO-basic

   Plasmid

   #196882

   Purpose

   Used for preparation of double-floxed inverse ORF vectors

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Type

   Empty backbone

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-1700029I15Rik-3×FLAG

   Plasmid

   #199689

   Purpose

   Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

   Insert

   1700029I15Rik (1700029I15Rik Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG.Myc-SARS2_3CL(C145A)

   Plasmid

   #200121

   Purpose

   Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   CAG

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG1.1-Tsks/1D4

    Plasmid

    #199623

    Purpose

    Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Tsks (variant X6) (Tsks Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS-mak10 gRNA

    Plasmid

    #160385

    Purpose

    Expresses guide RNA for targeting MAK10 in yeast

    Depositor

    John McCusker

    Insert

    mak10 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RNA pol III promoter (tRNA-Tyr)

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD70LacZ8.1-Pcadh-LacZ

    Plasmid

    #191624

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-3xHAtag-SP-CLSTN2

    Plasmid

    #191694

    Purpose

    For expression coding sequencing of CLSTN2

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of CLSTN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-SP-3xHAtag-PTPRO

    Plasmid

    #191695

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, full length coding sequence of PTPRO and 3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-ATG13 (1-197)

    Plasmid

    #188074

    Purpose

    To make ATG101/ATG13 HORMA complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized ATG13 (1-197)

    Promoter

    CMV

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mTurbo-mito

    Plasmid

    #187177

    Purpose

    Express mitochondira-localized miniTurbo under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

    Insert

    mtActA fused with miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-H2B-mTurbo

    Plasmid

    #187178

    Purpose

    Express histone H2B tagged with miniTurbo under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

    Insert

    histone H2B fused with miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPC-FLAG.Mm.EPC1.delta.EPcA

    Plasmid

    #184655

    Purpose

    Retroviral expression of mouse EPC1 delta EPcA

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    epc1 delta EPcA

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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