We narrowed to 8,639 results for: pcDNA3

1. pcDNA3-PBD-EYFP

   Plasmid

   #13723

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   PBD-EYFP (PAK1 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   p21 binding domain of human Pak1(amino acids 65-1…

   Available Since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. N370S GBA pcDNA3.1

   Plasmid

   #188583

   Purpose

   Ectopic expression N370S GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Mutation

   N370S

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PDE4D2cat-ICUE4

   Plasmid

   #181847

   Purpose

   ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
   

   Tags

   ECFP, PDE4D2(86-418), and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Hyg-mEGFP

   Plasmid

   #191847

   Purpose

   Expresses the monomeric form of EGFP

   Depositor

   Bertrand Collet

   Article

   Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

   Insert

   mEGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kohinoor-Vimentin/pcDNA3

   Plasmid

   #67772

   Purpose

   Vimentin-targeted Kohinoor for mammalian cell expression

   Depositor

   Takeharu Nagai

   Article

   Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

   Insert

   Kohinoor-Vimentin
   

   Tags

   Vimentin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-EGFP-Rac1(wt)

   Plasmid

   #13719

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   EGFP-Rac1(wt) (RAC1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-FluoSTEP-ICUE

   Plasmid

   #181845

   Purpose

   Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-ICUE (RAPGEF3 Human)
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ccdB-3xFLAG

   Plasmid

   #214984

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L444P GBA pcDNA3.1

   Plasmid

   #188582

   Purpose

   Ectopic expression L444P GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Mutation

   L444P

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Flag-gp130dimer

    Plasmid

    #174394

    Purpose

    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain with leucine zipper & C-C linker
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Flag-RNF5

    Plasmid

    #122043

    Purpose

    express RNF5

    Depositor

    Ze'ev Ronai

    Insert

    RNF5 (RNF5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-TDsmURFP-IRES-HO-1

    Plasmid

    #80346

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Expression

    Mammalian

    Promoter

    CMV and IRES

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. K44A HA-dynamin 2 pcDNA3.1

    Plasmid

    #34685

    Depositor

    Sandra Schmid

    Insert

    dynamin 2ba
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 236HSC WT (BRCA2)

    Plasmid

    #16246

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-myc3-CUL1

    Plasmid

    #19896

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 1 (CUL1 Human)
    

    Tags

    myc3

    Expression

    Mammalian

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2 TDP-43 5F-L YFP

    Plasmid

    #84914

    Purpose

    Mammalian expresion of TDP-43 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    F147L, F149L, F194L, F229L, F231L

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GGamma2-GFP2

    Plasmid

    #166774

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma2-GFP2 (GNG2 Human, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-ccdB-3xFLAG-V5

    Plasmid

    #87063

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-flag-YTHDF2

    Plasmid

    #52300

    Purpose

    full length YTHDF2 with N-terminal flag tag for mammalian cell expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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