165,666 results

1. pM1s3AsG

   Plasmid

   #137921

   Purpose

   E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19/PLIN2-GFP

   Plasmid

   #202194

   Purpose

   knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP knock-in

   Promoter

   lac

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Myc-IQGAP1

   Plasmid

   #30118

   Depositor

   David Sacks

   Article

   Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. LRG2.1_Neo

   Plasmid

   #125593

   Purpose

   Lentiviral expression of sgRNA with GFP and neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-CASANOVA

   Plasmid

   #113035

   Purpose

   CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB327

   Plasmid

   #113671

   Purpose

   expresses Ulp1_R3 in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Ulp1_R3
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   contains solubility enhancing mutations

   Promoter

   T7

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5 beta4

   Plasmid

   #16037

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. THBS1-bio-His

   Plasmid

   #53417

   Purpose

   Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   THBS1 (THBS1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-BAZ1B

   Plasmid

   #65372

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1B (BAZ1B Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PEmaxNG-_2star_IVT

    Plasmid

    #227678

    Purpose

    plasmid for SpCas9-NG-PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    NG-PEmax_2_star
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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