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pLKO.1-puro-shRPS6

Plasmid

#125780

Purpose

constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRPS6 (RPS6 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLL2 A10.5 gRNA

Plasmid

#90663

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLL2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLL2 (Guide Designation A10.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.773_774insH

Plasmid

#82855

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Additional H residue inserted between AA 773 and …

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-NSP4SA11

Plasmid

#89171

Purpose

Express Rotavirus SA11 NSP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP4

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180590

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Mutation

PHR(E281A)

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_53BP1_FLAG

Plasmid

#237345

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Insert

53BP1 (TP53BP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX-307_LgBiT

Plasmid

#191211

Purpose

Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

LgBiT (large bit)

Use

Lentiviral and Luciferase

Mutation

AB030246.1 (mutated in PMID 26569370)

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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