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We narrowed to 14,473 results for: cas9

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Showing: 2921 - 2960 of 14473 results

  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid
    #129053
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129053-plasmid-map-sequence-id-256238

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  3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129029
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129029-plasmid-map-sequence-id-256133

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  4. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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  6. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

    Plasmid
    #121812
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121812-plasmid-map-sequence-id-237358

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  10. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-GFP

    Plasmid
    #255027
    Purpose
    Lentiviral plasmid designed to express a SaCas9 compatible gRNA expression cassette and dSaCas9-KRAB-T2A-GFP protein; Similar to Plasmid #162334, but with GFP instead of Puro
    Depositor
    Jonathan Ploski
    Insert
    dSaCas9
    Use
    CRISPR and Lentiviral
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255027-plasmid-map-sequence-id-509438

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  21. pEvolvR-mCherry-Sniper-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249071
    Purpose
    Expresses nucleus-localized Sniper-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Sniper-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249071-plasmid-map-sequence-id-496323

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  22. pEvolvR-mCherry-SuperFi-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249070
    Purpose
    Expresses nucleus-localized SuperFi-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SuperFi-nCas9-PolI5M?
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249070-plasmid-map-sequence-id-496322

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  23. pEvolvR-mCherry-HSC1.2-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249073
    Purpose
    Expresses nucleus-localized HSC1.2-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HSC1.2-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249073-plasmid-map-sequence-id-496319

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  24. pEvolvR-mCherry-LZ3-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249072
    Purpose
    Expresses nucleus-localized LZ3-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    LZ3-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249072-plasmid-map-sequence-id-496318

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  25. pEvolvR-mCherry-HF1-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249075
    Purpose
    Expresses nucleus-localized HF1-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HF1-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249075-plasmid-map-sequence-id-496315

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  26. pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249076
    Purpose
    Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Hypa-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249076-plasmid-map-sequence-id-496314

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  27. pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249074
    Purpose
    Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SpRY-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249074-plasmid-map-sequence-id-496313

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  28. lenti-EF1a-dCas9-VPR-H2B-GFP

    Plasmid
    #242168
    Purpose
    Lentiviral expression of dCas9-VPR with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64-p65/RelA-Rta; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242168-plasmid-map-sequence-id-496079

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  29. lenti-EF1a-dCas9-VP64-H2B-GFP

    Plasmid
    #242164
    Purpose
    Lentiviral expression of dCas9-VP64 with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242164-plasmid-map-sequence-id-496078

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  30. lenti-EF1a-dCas9-VP64-H2B-mCherry

    Plasmid
    #242165
    Purpose
    Lentiviral expression of dCas9-VP64 with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242165-plasmid-map-sequence-id-496077

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  31. lenti-EF1a-dCas9-VPR-H2B-mCherry

    Plasmid
    #242169
    Purpose
    Lentiviral expression of dCas9-VPR with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64-p65/RelA-Rta; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242169-plasmid-map-sequence-id-496075

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  32. lenti-EF1a-dCas9-KRAB-H2B-mCherry

    Plasmid
    #242167
    Purpose
    Lentiviral expression of dCas9-KRAB with with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242167-plasmid-map-sequence-id-496074

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  33. lenti-EF1a-dCas9-KRAB-H2B-GFP

    Plasmid
    #242166
    Purpose
    Lentiviral expression of dCas9-KRAB with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242166-plasmid-map-sequence-id-496073

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  34. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

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  36. pAAV-synmiR-L-mRuby-Pro-dCas9-EGFP-4×17

    Plasmid
    #218268
    Purpose
    Expresses dCas9-EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-dCas9-EGFP-MREL_4×17
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218268-plasmid-map-sequence-id-494321

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  37. pAAV-synmiR-L-mRuby-Pro-dCas9-EGFP-4×19

    Plasmid
    #218270
    Purpose
    Expresses dCas9-EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-dCas9-EGFP-MREL_4×19
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218270-plasmid-map-sequence-id-494320

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  38. pGGB-OCS terminator-SunTag-HA-dCas9-NLS

    Plasmid
    #246798
    Purpose
    A GreenGate entry vector containing NLS-dCas9-HA-SunTag fused gene followed by a OCS terminator
    Depositor
    Danhua Jiang
    Insert
    NLS-dCas9-HA-SunTag-OCS terminator
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246798-plasmid-map-sequence-id-494307

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  40. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid
    #242664
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242664-plasmid-map-sequence-id-488971

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Showing: 2921 - 2960 of 14473 results