We narrowed to 2,349 results for: 683

1. mApple-M1 Spastin

   Plasmid

   #134461

   Purpose

   M1 Spastin expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met to Ala change at amino acid 87

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Parkin

   Plasmid

   #187897

   Purpose

   Mammalian cell expression of untagged Parkin

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   PRKN (PRKN Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1-USP11

   Plasmid

   #78849

   Purpose

   To purify GST-tagged recombinant USP11 protein

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

   Insert

   USP11 (USP11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

   Plasmid

   #134864

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   emerin (EMD Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

   Plasmid

   #169921

   Purpose

   DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   IRES-Puro-pEF1a-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR v2 hBAK

   Plasmid

   #129579

   Purpose

   CRISPR deletion of human BAK

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs human BAK (BAK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-MBP-NRBF2

   Plasmid

   #99330

   Purpose

   Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

   Insert

   Nuclear receptor-binding factor 2 (NRBF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Per2:iLuc

   Plasmid

   #160293

   Purpose

   target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

   Depositor

   Joseph Takahashi

   Article

   Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

   Insert

   Per2LUC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   no promotor

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-Rpp29-C1

   Plasmid

   #134545

   Purpose

   Expresses YFP-Rpp29 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp29 (POP4 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ-dpcoCas9-Act3.0

    Plasmid

    #158408

    Purpose

    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-Neuromodulin-IRES-Puromycin

    Plasmid

    #134666

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Neuromodulin (GAP43 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-M1 SpastinE442Q

    Plasmid

    #134464

    Purpose

    M1 SpastinE442Q expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Glu to Gln at amino acid 442

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB2_mScarletI_mVenusNB-MrkHR113A

    Plasmid

    #182292

    Purpose

    expresses c-di-GMP mutant biosensor in E. coli

    Depositor

    Howard Berg

    Article

    Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

    Insert

    mVenusNB-MrkHR113A
    

    Use

    Tags

    mVenusNB internal fusion to MrkH with a R113A poi…

    Expression

    Bacterial

    Mutation

    changed arginine 113 to alanine, MrkH numbering …

    Promoter

    proC

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNAIIIB cdc42 QL

    Plasmid

    #61636

    Purpose

    mammalian expression of cdc42 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. YF-Cdc42(CA)

    Plasmid

    #20152

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Cdc42(CA) (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cdc42H was inserted into the multiple cloning sit…

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-EGFP-NEMO-D304N

    Plasmid

    #187898

    Purpose

    Mammalian cell expression of D304N mutation in NEMO

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    HA-EGFP-NEMO-D304N (Ikbkg Mouse)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    D304N

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQStrep2-ARPC3

    Plasmid

    #31580

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mApple-M1 SpastinK388R

    Plasmid

    #134463

    Purpose

    M1 SpastinK388R expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys to Arg at amino acid 388

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDY118A

    Plasmid

    #182958

    Purpose

    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Inserts

    Cas9
    Gam, Beta, Exo
    I-scel
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge