We narrowed to 5,818 results for: pCas

1. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

   Plasmid

   #197421

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

   Plasmid

   #197422

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

   Plasmid

   #197423

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

   Plasmid

   #197424

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

   Plasmid

   #197425

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

   Plasmid

   #197426

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas-Cas12m-ΔZF

   Plasmid

   #192276

   Purpose

   Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   Cas12m ΔZF
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   H459A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas-dCas12m-ΔZF

   Plasmid

   #192277

   Purpose

   pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m ΔZF
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A, H549A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-DIO-basic

   Plasmid

   #196882

   Purpose

   Used for preparation of double-floxed inverse ORF vectors

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Type

   Empty backbone

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-1700029I15Rik-3×FLAG

    Plasmid

    #199689

    Purpose

    Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

    Insert

    1700029I15Rik (1700029I15Rik Mouse)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG.Myc-SARS2_3CL(C145A)

    Plasmid

    #200121

    Purpose

    Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    CAG

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG1.1-Tsks/1D4

    Plasmid

    #199623

    Purpose

    Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Tsks (variant X6) (Tsks Mouse)
    

    Tags

    1D4

    Promoter

    CAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAS-mak10 gRNA

    Plasmid

    #160385

    Purpose

    Expresses guide RNA for targeting MAK10 in yeast

    Depositor

    John McCusker

    Insert

    mak10 gRNA
    

    Expression

    Yeast

    Promoter

    RNA pol III promoter (tRNA-Tyr)

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXD70LacZ8.1-Pcadh-LacZ

    Plasmid

    #191624

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-3xHAtag-SP-CLSTN2

    Plasmid

    #191694

    Purpose

    For expression coding sequencing of CLSTN2

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of CLSTN2
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-SP-3xHAtag-PTPRO

    Plasmid

    #191695

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, full length coding sequence of PTPRO and 3xHAtag
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-ATG13 (1-197)

    Plasmid

    #188074

    Purpose

    To make ATG101/ATG13 HORMA complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Expression

    Mammalian

    Mutation

    codon-optimized ATG13 (1-197)

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-mTurbo-mito

    Plasmid

    #187177

    Purpose

    Express mitochondira-localized miniTurbo under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

    Insert

    mtActA fused with miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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