We narrowed to 2,601 results for: ubiquitin

1. pETDUET-GST AO7RE P199A

   Plasmid

   #139427

   Purpose

   GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed proline 199 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDUET-GST AO7RE I137A

   Plasmid

   #139319

   Purpose

   GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed isoleucine 137 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223 LC3B Q116P G120

   Plasmid

   #129291

   Purpose

   Gateway entry clone encoding human deconjugation-resistant LC3B

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-HA-RNF68 [dRING]

   Plasmid

   #126549

   Purpose

   Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF68 (PCGF1 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   RING domain deletion mutant [dRING mutation is a …

   Promoter

   LTR

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL I3

   Plasmid

   #113514

   Purpose

   Expression of I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFL His-I3 V41A / W43A

   Plasmid

   #113515

   Purpose

   Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   V41A /W43A

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-6P-1 p37deltaSEP

   Plasmid

   #113501

   Purpose

   Bacterial expression of GST-tagged p37 lacking the SEP domain

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p37 (Ubxn2b Mouse)
   

   Use

   Tags

   GST-PreScission protease site

   Expression

   Bacterial

   Mutation

   delta 140-206

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-6P-1 p37delta2-81

   Plasmid

   #113502

   Purpose

   Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p37 (Ubxn2b Mouse)
   

   Use

   Tags

   GST-PreScission protease site

   Expression

   Bacterial

   Mutation

   delta 2-81

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFL His-I3

   Plasmid

   #113513

   Purpose

   Expression of His-I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p7054 pHAGE-P-CMVt-N-HA-GAW-COPE

    Plasmid

    #100144

    Purpose

    Expresses COPE

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    COPE (COPE Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6

    Plasmid

    #100140

    Purpose

    Encodes amino acids 4421-4834 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 4421-4834

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5

    Plasmid

    #100139

    Purpose

    Encodes amino acids 3550-4500 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 3550-4500

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4

    Plasmid

    #100138

    Purpose

    Encodes amino acids 2600-3600 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 2600-3600

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2

    Plasmid

    #100136

    Purpose

    Encodes amino acids 950-1750 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 950-1750

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p6325 pHAGE-P-CMVt-N-HA-GAW-HERC2-F1

    Plasmid

    #100135

    Purpose

    Encodes amino acids 1-1000 of HERC2.

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HERC2 (HERC2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acids 1-1000

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-CD4-YEML-myc

    Plasmid

    #58538

    Purpose

    Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

    Expression

    Mammalian

    Mutation

    Added C-terminal Tyrosine-Glutamate-Methionine-Le…

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1/GS-DJ1-E18D-V5

    Plasmid

    #29346

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    V5 epitope

    Expression

    Mammalian

    Mutation

    E18D

    Promoter

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1/GS-DJ1-E18N-V5

    Plasmid

    #29341

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    V5 epitope

    Expression

    Mammalian

    Mutation

    Glu18Asn

    Promoter

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pUltra-puro-RTTA3

    Plasmid

    #58750

    Purpose

    silence doxycyclin inducible pUltra-dox

    Depositor

    Kitai Kim, Yildirim Dogan

    Insert

    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Ubiquitin C promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-HA-UBE2L3

    Plasmid

    #27561

    Depositor

    Shao-Cong Sun

    Insert

    UBE2L3 (UBE2L3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge