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pGGD001

Plasmid

#48833

Purpose

Provides linker:GFP as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

Linker:GFP

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

Plasmid

#210709

Purpose

This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN

Use

AAV and CRISPR

Expression

Mammalian

Mutation

N55K in MCP

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR

Plasmid

#116731

Purpose

Lentiviral expression of EGFR

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHaMDRwt

Plasmid

#10957

Depositor

Michael Gottesman

Article

Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

Insert

MDR1 (ABCB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP_mEGFP-GluA1c

Plasmid

#114136

Purpose

Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mEGFP tagged GluA1 C-terminus

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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spNW15

Plasmid

#173483

Purpose

Expresses spNW15 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW15

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-MCC

Plasmid

#133307

Purpose

"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

microcin-V bacteriocin cassette (cvaC E. coli)

Use

Synthetic Biology

Expression

Bacterial

Mutation

N112D (please see depositors comments)

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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p23-mNeongreen-DDX21 WT-mRuby3

Plasmid

#179225

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mNeongreen and mRuby3

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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