163,354 results

1. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

   Plasmid

   #50460

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. PA-GFP-α-Tubulin

   Plasmid

   #213900

   Purpose

   Imaging of Tubulin using Photoactivatable GFP

   Depositor

   Lukas Kapitein

   Insert

   PA-GFP-α-Tubulin
   

   Use

   Tags

   Photoactivatable GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2939

   Plasmid

   #144415

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXA6 (HOXA6 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21 gamma-tubulin

   Plasmid

   #101825

   Purpose

   production of gamma-tubulin in bacteria

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Whole protein

   Promoter

   T7

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNBU2_repA2

   Plasmid

   #211911

   Purpose

   Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

   Depositor

   Adam Arkin

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-puro-2A-TEVp

   Plasmid

   #99610

   Purpose

   Lentivirus expressing puromycin resistance gene and TEV protease

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   Puromycin resistance (PAC gene)
   TEV protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINF

   Plasmid

   #26042

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His3C-lacZ
   

   Use

   Tags

   MAHHHHHHSSGLEVL

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHTN-HaloTag_RPA2:1-270

    Plasmid

    #211013

    Purpose

    Mammalian expression of full-length human RPA2. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RPA2:M1-E270 (RPA2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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