We narrowed to 10,333 results for: SEC

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGAm (FlincG3)

Plasmid

#49202

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG3

Tags

His tag + other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Met to Lys in cpEGFP region similar to CGaMP3

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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EK0479 CMV-TO-VEGF-A(165) (FLP-IN)

Plasmid

#233384

Purpose

Secreted human VEGF-A

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGF-A (VEGFA Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKM7

Plasmid

#154164

Purpose

mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

mCherry

Expression

Insect

Promoter

IE1 promoter and hr5 enhancer from Autographa cal…

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-PE2-attB

Plasmid

#149550

Purpose

Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

UAS

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ac-AChBP

Plasmid

#63128

Purpose

Expression of Aplysia californica AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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