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pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-Fkbp1x 2A Hygro

Plasmid

#102806

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pRRLSIN.cPPT.PGK.MyoD.WPRE

Plasmid

#122053

Purpose

Lentiviral expression of MyoD

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

Plasmid

#20293

Purpose

Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Alanine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMN-326

Plasmid

#195451

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - minTK - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-325

Plasmid

#195450

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF1 BS - minTK - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3

Plasmid

#106115

Purpose

Lentiviral expression of PRKRA delta3

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK and myc

Expression

Mutation

Truncation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAPM sh2 DNMT1

Plasmid

#88885

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.2 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12

Plasmid

#106116

Purpose

Lentiviral expression of PRKRA delta 12

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

Expression

Mutation

Truncation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-THT III

Plasmid

#55791

Purpose

GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Type

Empty backbone

Use

RNAi; Gateway

Tags

Expression

Mammalian

Mutation

Promoter

modified H1 RNA gene

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 A114Q

Plasmid

#175767

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 A114Q

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

A114Q

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1aMS2p65HSF1hyg-W

Plasmid

#112920

Purpose

Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

MS2p65HSF1-T2Ahyg

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

Plasmid

#20295

Purpose

Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Threonine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pSLIK TT 3xFLAG Luciferase neo

Plasmid

#98392

Purpose

Lentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-HP1cs 2A Hygro

Plasmid

#102810

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-HP1cs fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-HP1cs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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FUp95bGW (B6)

Plasmid

#74015

Purpose

lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 beta isoform

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant Psd95

Promoter

hUbC

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUGW (B3)

Plasmid

#74012

Purpose

lentiviral expression of EGFP and Psd95 shRNA

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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