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pAVEC2_C-mCitrine

Plasmid

#231297

Purpose

For transiently expressing fusion proteins with C terminally tagged mCitrine in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mScarletI

Plasmid

#231298

Purpose

For transiently expressing fusion proteins with C terminally tagged mScarletI in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMRS-empty

Plasmid

#220203

Purpose

mini-Tn7 cloning vector - empty backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCM271rfp

Plasmid

#59774

Purpose

pCMrfp with TrfA mutation (271R to C)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

RFP

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB

Plasmid

#92234

Purpose

Express wild type prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

none

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUdOm1.1

Plasmid

#210273

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP47-2A(sfGFP)

Plasmid

#126991

Purpose

Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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KO plasmid

Plasmid

#135970

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pUdOm2.1

Plasmid

#210278

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-His10Smt3-gamma-toxin

Plasmid

#197000

Purpose

Bacterial expression plasmid for gamma toxin tRNA endonuclease

Depositor

Dragony Fu

Article

Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

Insert

Gamma toxin

Tags

His10-Smt3

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pkk223_Null

Plasmid

#213111

Purpose

pkk223 backbone plasmid

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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EMM65

Plasmid

#49042

Purpose

Backbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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EMM67

Plasmid

#49043

Purpose

Backbone contains SHD/G 0.5 Domain TALE N-terminal TALE C-terminus, LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pJUMP45-2A(sfGFP)

Plasmid

#126989

Purpose

Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CMV mCherry-eGFP BGH

Plasmid

#92280

Purpose

Control FRET construct; tandem mCherry-eGFP fusion

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

mCherry-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLXMA_RK2 L2A

Plasmid

#153361

Purpose

Expression vector for plants with low-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6 1397N

Plasmid

#221939

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA11 1397N

Plasmid

#221940

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm6.1

Plasmid

#210286

Purpose

Empty backbone with trimethoprim resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJYDN3

Plasmid

#162456

Purpose

Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pJYP1

Plasmid

#202406

Purpose

Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

Depositor

Remy Loris

Article

Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

Type

Empty backbone

Expression

Bacterial

Promoter

fdeA

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pScalps hygro

Plasmid

#184785

Purpose

Empty Lentiviral vector backbone

Depositor

Ajit Chande

Article

Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

Type

Empty backbone

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUdOm2.4

Plasmid

#210281

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CMV NAPA-A BGH

Plasmid

#92277

Purpose

FRET probe; extracellular membrane tethered eGFP

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Astrocyte

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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