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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI;

Showing: 2961 - 3000 of 8358 results

  1. Ec holA-pET(11a)(ACH-)

    Plasmid
    #237233
    Purpose
    over-express E. coli holA (Pol III delta subunit)
    Depositor
    Michael O'Donnell
    Insert
    holA (delta) (holA E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237233-plasmid-map-sequence-id-469254

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  2. pSG7084 pG46-46

    Plasmid
    #232621
    Purpose
    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).
    Depositor
    Stephan Gruber
    Type
    Empty backbone
    Use
    Precursor plasmid to generate modified dna circles
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    232621-plasmid-map-sequence-id-468938

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  3. EP001: pLenti CMV Blast::eGFP-RC-I-1-H11

    Plasmid
    #209301
    Purpose
    Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal eGFP
    Depositor
    Adam Cohen
    Insert
    RC-I-1-H11
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209301-plasmid-map-sequence-id-414434

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  4. EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11

    Plasmid
    #209302
    Purpose
    Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal HaloTag
    Depositor
    Adam Cohen
    Insert
    RC-I-1-H11
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209302-plasmid-map-sequence-id-414430

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  5. pBHM2643

    Plasmid
    #229527
    Purpose
    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    Tags
    t2a-mNeonGreen-mAID_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    229527-plasmid-map-sequence-id-459548

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  6. pAG Retro Neo Empty

    Plasmid
    #234460
    Purpose
    Retroviral vector for cloning and expressing your gene of interest. Neomycin selection.
    Depositor
    Addgene Research Program, Melina Fan
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234460-plasmid-map-sequence-id-464633

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  7. Francisella tularensis enoyl reductase

    Plasmid
    #231104
    Purpose
    Expresses FabI under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    Enoyl-acyl carrier protein reductase
    Use
    Tags
    6x HIS, GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231104-plasmid-map-sequence-id-465515

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  8. mP_ILB_Vector 1

    Plasmid
    #232193
    Purpose
    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
    Depositor
    Matrika Bhattarai
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232193-plasmid-map-sequence-id-463610

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  9. FtHAP-pNIC-Bsa4

    Plasmid
    #86778
    Purpose
    Expresses Francisella tularensis Histidine Acid Phosphatase under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    Histidine Acid Phosphatase
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    86778-plasmid-map-sequence-id-462633

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  10. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  11. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid
    #220961
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    220961-plasmid-map-sequence-id-437427

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  12. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Stephen Benkovic
    Insert
    uvsY (uvsY Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228214-plasmid-map-sequence-id-455995

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  14. AAV-SYN-mCherry-oPRE

    Plasmid
    #218682
    Purpose
    AAV vector plasmid expressing mCherry under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mCherry
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218682-plasmid-map-sequence-id-447609

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  15. GPD1_SB

    Plasmid
    #224147
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-1 (gpd-1 Nematode)
    Use
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224147-plasmid-map-sequence-id-444366

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  16. ADH1_KW

    Plasmid
    #224149
    Purpose
    IPTG-inducible expression of C. elegans protein ADH-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    ADH-1 (sodh-1 Nematode)
    Use
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224149-plasmid-map-sequence-id-444365

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  17. GPD3_KW

    Plasmid
    #224151
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-3 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-3 (gpd-3 Nematode)
    Use
    Tags
    intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224151-plasmid-map-sequence-id-444362

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  18. WT_H1-S1

    Plasmid
    #218400
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    218400-plasmid-map-sequence-id-442676

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  19. pcDNA-DDX53-FLAG

    Plasmid
    #222241
    Purpose
    Express FLAG tagged DDX53 in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    222241-plasmid-map-sequence-id-441350

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  20. pET29a-DDX53-Q249A

    Plasmid
    #222216
    Purpose
    Express DDX53 mutant Q249A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_Q249A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222216-plasmid-map-sequence-id-441427

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  21. pET29a-DDX53-K272A

    Plasmid
    #222215
    Purpose
    Express DDX53 mutant K272A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_K272A (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222215-plasmid-map-sequence-id-441662

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  22. pET29a-DDX53-E377Q

    Plasmid
    #222217
    Purpose
    Express DDX53 mutant E377Q protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_E377Q (DDX53 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    E377Q
    Promoter
    T7
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222217-plasmid-map-sequence-id-441653

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  23. pcDNA-DDX53-GFP

    Plasmid
    #222239
    Purpose
    Express GFP tagged DDX53 in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222239-plasmid-map-sequence-id-441404

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  24. pcDNA-DDX53-K272A

    Plasmid
    #222242
    Purpose
    Express FLAG tagged DDX53 mutant K272A in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K272A
    Promoter
    T7
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222242-plasmid-map-sequence-id-441373

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  25. WT_S1-H1

    Plasmid
    #218392
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218392-plasmid-map-sequence-id-436407

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  26. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  27. MUT_S1-N

    Plasmid
    #218398
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218398-plasmid-map-sequence-id-433922

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  28. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218397-plasmid-map-sequence-id-433918

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  29. WT_S7-H1

    Plasmid
    #218393
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218393-plasmid-map-sequence-id-433906

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  30. pR6K-GFP-CASTIF

    Plasmid
    #199653
    Purpose
    R6K plasmid encoding a type I-F CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199653-plasmid-map-sequence-id-412253

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  31. pR6K-GFP-CASTV

    Plasmid
    #199657
    Purpose
    R6K plasmid encoding a type V CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST V systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199657-plasmid-map-sequence-id-412267

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  32. pCLX-KRT7-GFP

    Plasmid
    #216853
    Purpose
    This plasmid is for the production of lentiviral vectors that can be used to identify and live sort pancreatic progenitor expresssing the Keratin7 gene.
    Depositor
    Patrick Salmon
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Keratin7
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216853-plasmid-map-sequence-id-431654

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  33. pET-C

    Plasmid
    #213496
    Purpose
    Encodes Node C of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    lambda cI 5G6GP
    mCherry
    Use
    Synthetic Biology
    Tags
    ASV tag and LVA tag
    Expression
    Mutation
    Promoter
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    213496-plasmid-map-sequence-id-430629

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  34. HA Display Part 1-4 DO

    Plasmid
    #206514
    Purpose
    CcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    206514-plasmid-map-sequence-id-424195

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  35. pCAG-ORF3cStrep

    Plasmid
    #208728
    Purpose
    Expresses ORF3c-Strep in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Use
    Tags
    Strep
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    208728-plasmid-map-sequence-id-420909

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  36. p_L_3c_SG_RLuc

    Plasmid
    #208729
    Purpose
    For assessing leaky scanning rates in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Use
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    208729-plasmid-map-sequence-id-420912

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  37. pHflu3

    Plasmid
    #203881
    Purpose
    pHI1 with insertion of kanamycin-resistance gene
    Depositor
    Bogumil Karas
    Insert
    aminoglycoside O-phosphotransferase APH(3')-IIa
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203881-plasmid-map-sequence-id-403829

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  38. pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

    Plasmid
    #204079
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204079-plasmid-map-sequence-id-413894

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  39. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

    Plasmid
    #198069
    Purpose
    Mammalian expression of iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198069-plasmid-map-sequence-id-389877

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  40. pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

    Plasmid
    #198062
    Purpose
    The lentiviral vector for iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198062-plasmid-map-sequence-id-389830

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Showing: 2961 - 3000 of 8358 results