We narrowed to 1,606 results for: Sp1

1. Flag-M4-AR-S81A

   Plasmid

   #171241

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Ser81Ala (AR-S81A)

   Promoter

   CMV

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hAR-L26A,F27A,W433A,L436A,F437A

   Plasmid

   #89085

   Purpose

   mammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifs

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   hAR FxxLF WxxLF mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-L26A,F27A,W433A,L436A,F437A

   Promoter

   CMV

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pCIneo-AR-S256A

   Plasmid

   #171227

   Purpose

   Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)

   Depositor

   Steven Balk

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

   Insert

   human androgen receptor Ser256Ala mutant (AR Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   AR-Ser256Ala (AR-S256A)

   Promoter

   CMV

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIneo-AR-S308A

   Plasmid

   #171229

   Purpose

   Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)

   Depositor

   Steven Balk

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

   Insert

   human androgen receptor Ser308Ala mutant (AR Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   AR-Ser308Ala (AR-S308A)

   Promoter

   CMV

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIneo-AR-S90A

   Plasmid

   #171225

   Purpose

   Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)

   Depositor

   Steven Balk

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

   Insert

   human androgen receptor Ser90Ala mutant (AR Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   AR-Ser90Ala (AR-S90A)

   Promoter

   CMV

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ARR3tk-eGFP/SV40-mCherry

   Plasmid

   #132360

   Purpose

   To measure transcriptional activity of Androgen Receptor

   Depositor

   Charles Sawyers

   Article

   Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

   Insert

   AR responsive elements (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRR-AR

   Plasmid

   #73045

   Purpose

   Constitutively expresses the androgen receptor, which is required for the activation of the promoter in pLAREG (Addgene plasmid #73045)

   Depositor

   David McMillen

   Article

   Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

   Insert

   Androgen receptor (AR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   please see depositor comments below

   Promoter

   glyceraldehyde phosphate dehydrogenase (GPD)

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3007

   Plasmid

   #144483

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMAD1 (SMAD1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAL-hAR-658-919

   Plasmid

   #89082

   Purpose

   mammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

   Insert

   hAR-658-919 (AR Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 658-919

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LT3GEPIR-ARshRNA2

    Plasmid

    #220562

    Purpose

    To inducibly knockdown AR expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    AR shRNA2 (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT3GEPIR-ARshRNA1

    Plasmid

    #220561

    Purpose

    To inducibly knockdown AR expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    AR shRNA1 (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-15b-hAR-663-919

    Plasmid

    #89083

    Purpose

    bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

    Insert

    hAR ligand binding domain (AR Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 663-919

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO HIS HSPH2

    Plasmid

    #19535

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid

    #110626

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDragon-Ctgf

    Plasmid

    #155015

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Ccn2 (Ccn2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    M24T (please see depositor comments)

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh55

    Plasmid

    #106728

    Purpose

    expression of gRNA targeting B4GALT7

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B4GALT7 (B4GALT7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

17. Frt-hspB1

    Plasmid

    #63092

    Purpose

    Expression of HSP1 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-M4-AR-W741C

    Plasmid

    #171247

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys (AR-W741C)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-M4-AR-S81D

    Plasmid

    #171242

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser81Asp (AR-S81D)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid

    #59826

    Purpose

    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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