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mTFP1-MCHR1-N-10

Plasmid

#55499

Purpose

Localization: Cilium, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

MCHR1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

Plasmid

#233353

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP

Plasmid

#233357

Purpose

CMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKC-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUltra

Plasmid

#24129

Purpose

3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

Depositor

Malcolm Moore

Article

Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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Cyto-roGFP

Plasmid

#49435

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

roGFP2

Use

Adenoviral

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCfB2188-PrARG8-GFP::URA3

Plasmid

#216512

Purpose

Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrARG8-yeGFP

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

ARG8

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB2197-PrGNP1-mK2-NatMX

Plasmid

#216513

Purpose

Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrGnp1-mKate2

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

GNP1

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Cav1-GFP

Plasmid

#14433

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

EGFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pVSVΔG-eGFP-linker

Plasmid

#231707

Purpose

Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

Depositor

Ron Geller

Article

Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

Insert

VSV antigenome

Use

Vsv expression

Expression

Mammalian

Mutation

ΔG (VSV glycoprotein removed)

Promoter

T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9

Plasmid

#46920

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMetYCgate

Plasmid

#105074

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

Cub-PLV

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pNXgate33-3HA

Plasmid

#105075

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-Tev

Plasmid

#177142

Purpose

Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

Tev

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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mActA-FKBP-mCherry

Plasmid

#210295

Purpose

ActuAtor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-FKBP-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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