We narrowed to 1,720 results for: plasmids pcas

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AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Expression

Mammalian

Promoter

U1A

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLC133

Plasmid

#198639

Purpose

Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

dCas9

Tags

3x-FLAG

Expression

Bacterial

Promoter

pTet

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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BPK1169: CAG-DmrC-NLS-3xFLAG-P65

Plasmid

#104564

Purpose

Mammalian expression vector for DmrC fused to p65 activation domain

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-p65

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLP857_α-syn:oScarlet KI donor

Plasmid

#239403

Purpose

AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

oScarlet

Use

AAV and CRISPR

Tags

oScarlet

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM01

Plasmid

#233473

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM03

Plasmid

#233475

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR0-37a +SV40 base prom-GFP-IRES-AP

Plasmid

#225889

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR0-37a

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR3-17c +SV40 base prom-GFP-IRES-AP

Plasmid

#225890

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17c

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR3-17d +SV40 base prom-GFP-IRES-AP

Plasmid

#225891

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17d

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Cas9-P2A-dTomato-PS9

Plasmid

#231911

Purpose

For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

Cas9-P2A-dTomato

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Cas9-P2A-dTomato-T20

Plasmid

#231912

Purpose

For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

Cas9-P2A-dTomato

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pWN10042

Plasmid

#89052

Purpose

GF-ori-FP plasmid for GFxFP assay

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

EGFP part 1
EGFP part 2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and none

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.GFP

Plasmid

#57822

Purpose

3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
eGFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJT166_GalL_A3A-BE3

Plasmid

#145116

Purpose

Expressing base editor A3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJT82_GalL_nCDA1Δ198-BE3

Plasmid

#145046

Purpose

Expresses nCDA1Δ198-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ198-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-198AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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