We narrowed to 1,661 results for: sp1

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TFORF0192

Plasmid

#141549

Purpose

Lentiviral vector for overexpressing the RFX4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RFX4 (RFX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP+AAVA)

Plasmid

#14955

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP+AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
p264 pSP72 hAR-LBD(LNCaP)

Plasmid

#12495

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

AR LBD (AR Human)

Expression

Bacterial

Mutation

Thr 878 Ala in AR-LBD

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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p260 pCMV-CRE-M-AR(wt)

Plasmid

#12494

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

CRE-M and AR ligand binding domain

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1 ASA

Plasmid

#13479

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1ASA (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Arginine 53 to Alanine, Changed Arginine…

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pet151D-TOPO BROX 1-411;

Plasmid

#89602

Purpose

Expresses full length human Brox in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Tags

His Tag, V5 tag

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
MIS18A_pLENTI-CAG-IRES-GFP

Plasmid

#176996

Purpose

Mammalian lentiviral expression vector encoding MIS18A

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MIS18A (MIS18A Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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p270 pCMV-CRE-M-AR(LNCaP)

Plasmid

#12496

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

Thr 868 Ala in AR-LBD

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-hAR-1-660

Plasmid

#89110

Purpose

mammalian expression of human androgen receptor amino acid residues 1-660, insert can be excised with EcoRI/Xba or BamHI

Depositor

Elizabeth Wilson

Article

Langley et al J Biol Chem. 1995 Dec 15;270(50):29983-9

Insert

hAR-1-660 (AR Human)

Expression

Mammalian

Mutation

contains aa 1-660

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1L16A/L17A

Plasmid

#13478

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A/L17A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine; changed Leucine 1…

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase S1226A/ C2029A

Plasmid

#59823

Purpose

Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

S1226A/ C2029A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1 Catalytic Mutant

Plasmid

#48971

Purpose

Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566; Catalytic Mutant C464A

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only