We narrowed to 6,298 results for: tev

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pGEX-4T1-GST-TEV-NAP1

Plasmid

#217610

Purpose

For expression in E. coli of NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

GST

Expression

Bacterial

Promoter

TAC promoter

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV and mCherry

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO5(wt)

Plasmid

#191803

Purpose

Full length wt AtPCO5 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

Plasmid

#186274

Purpose

Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas6-RT-Cas1

Tags

His6-MBP

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pnEA-vH_His-TEV-DSep1-msfGFP

Plasmid

#174494

Purpose

bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

Sep1 (Sep1 Fly)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dH85E-cs-TEV-STREP

Plasmid

#171838

Purpose

Mammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

H85E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK88E-cs-TEV-STREP

Plasmid

#171839

Purpose

Mammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K88E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dI92E-cs-TEV-STREP

Plasmid

#171840

Purpose

Mammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

I92E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dC93E-cs-TEV-STREP

Plasmid

#171841

Purpose

Mammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

C93E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171842

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

R108E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171843

Purpose

Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

R125E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171844

Purpose

Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep and mCherry

Expression

Mammalian

Mutation

K128E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-TEV-Gly-RadA∆C46

Plasmid

#128702

Purpose

Expresses PhoRadA intein split at residue 126 from the N-terminal

Depositor

Sharon Rozovsky

Article

Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

Insert

RadA

Tags

Hisx6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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His8:MBP-tev-Ala-eK-Flv

Plasmid

#83393

Purpose

bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

Depositor

Nico Dissmeyer

Article

Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

Insert

flavin binding protein MioC

Use

Synthetic Biology

Tags

3xHA, 8xHis, and MBP

Expression

Bacterial

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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His8:MBP-tev-Ile-eK-Flv

Plasmid

#83392

Purpose

bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

Depositor

Nico Dissmeyer

Article

Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

Insert

flavin binding protein MioC

Use

Synthetic Biology

Tags

3xHA, 8xHis, and MBP

Expression

Bacterial

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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