162,999 results

1. pEGFP-Nup88

   Plasmid

   #64283

   Purpose

   GFP-tagged mammalian expression of full-length Nup88

   Depositor

   Birthe Fahrenkrog

   Article

   Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

   Insert

   Nup88 (NUP88 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. p101_CRISPRai_PiggyBac

   Plasmid

   #213777

   Purpose

   Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   VPR-dSaCas9
   dSpCas9-KRAB-BFP
   zeocin
   Tet-On transactivator
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a (EF-1-alpha intron a) and Tet_On_TRE

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-SaCas9-U6-sgGria1

   Plasmid

   #124853

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-mKate2-P2A-TurboID-NS

   Plasmid

   #166568

   Purpose

   Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   mKate2-P2A-TurboID
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-FOXO3

   Plasmid

   #153142

   Purpose

   Expression of Flag tagged human FOXO3 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXO3 (FOXO3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Actin-mRFP-PAGFP

   Plasmid

   #62382

   Purpose

   Expresses beta actin with both a PAGFP and mRFP tag.

   Depositor

   Tim Mitchison, Guillaume Charras

   Article

   Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

   Insert

   beta actin (ACTB Human)
   

   Use

   Tags

   PAGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-CMV-Galpha-s ONE-GO

   Plasmid

   #189731

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-s ONE-GO
   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. HA-EGFP-HaloTag2

    Plasmid

    #41742

    Depositor

    Craig Crews

    Article

    Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

    Insert

    Halotag 2
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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