162,999 results

1. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

   Plasmid

   #134860

   Purpose

   Expresses EGFP-tagged recycling endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB11B (RAB11B Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST-SorLA-v1

   Plasmid

   #154892

   Purpose

   Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   SORL1 (SORL1 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-Flpo

   Plasmid

   #60662

   Purpose

   Expresses pCAG-Flpo; codon-optimized for mammalian expression

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Flpo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Gi3-CASE

   Plasmid

   #168122

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A114/E115 within GNAI1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSV human p85 alpha (HA tag)

   Plasmid

   #11499

   Depositor

   Ronald Kahn

   Article

   Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

   Insert

   p85 alpha (PIK3R1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHLmMBP-10

   Plasmid

   #72348

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BRD4

   Plasmid

   #38942

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

   Plasmid

   #204694

   Purpose

   This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   direct fusion of BRD4 and NUT

   Promoter

   CMV

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p75-RFP

    Plasmid

    #24092

    Depositor

    Moses Chao

    Insert

    p75NTR (NGFR Human)
    

    Use

    Tags

    DSRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge