We narrowed to 875 results for: seph

1. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-human-BNIP3

   Plasmid

   #100781

   Purpose

   Mammalian expression of BNIP3

   Depositor

   Joseph Gordon

   Article

   Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

   Insert

   human-BNIP3 (BNIP3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-PB-SRT-tdTomato

   Plasmid

   #154889

   Purpose

   piggyBac self-reporting transposon with tdTomato marker in AAV backbone

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   piggyBac SRT
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpRYc

   Plasmid

   #175575

   Purpose

   Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

   Insert

   SpRYc
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   PID of SpRY grafted onto the N-terminal domain of…

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SiriusGFP

   Plasmid

   #123201

   Purpose

   We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

   Insert

   SiriusGFP
   

   Use

   Mouse Targeting

   Tags

   N/A

   Expression

   Bacterial and Mammalian

   Mutation

   eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

   Promoter

   CMV

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. sh-SRF

   Plasmid

   #100797

   Purpose

   expression of shRNA targeting SRF

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

   Insert

   rat SRF
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-sh-Nix

   Plasmid

   #100770

   Purpose

   Lentiviral expression of shRNA targeting Nix

   Depositor

   Joseph Gordon

   Article

   da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

   Insert

   Lenti-sh-Nix
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

   Plasmid

   #133789

   Purpose

   CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized CjeCas9
   

   Use

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_GW_Dest

   Plasmid

   #86955

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

   Depositor

   Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid

    #134331

    Purpose

    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized Nme2Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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