Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 155,982 results for: ins

Showing: 2251 - 2280 of 155982 results

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pMSCV-FKBP-ZC3H12B

Plasmid

#221300

Purpose

Expression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ZC3H12B (pLxIS)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-BR1

Plasmid

#192816

Purpose

Expresses BR1 in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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acvr1l-VAAA-HA

Plasmid

#201905

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Tags

HA

Mutation

T186V, S187A, S189A, S191A

Promoter

SP6

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA-CIM1-2mut_T

Plasmid

#147635

Purpose

Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6C_1207-1609-M2WA-CIM1-2mut (TNRC6C Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-V5His6_R

Plasmid

#147400

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_825-1384_N

Plasmid

#147063

Purpose

Insect Expression of DmGW182_825-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_825-1384 (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1-603_N

Plasmid

#147057

Purpose

Insect Expression of DmGW182_1-603

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_1-603 (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_1-634_N

Plasmid

#147058

Purpose

Insect Expression of DmGW182_1-634

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_1-634 (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6Biso1_1-1619_L

Plasmid

#146848

Purpose

Insect Expression of HsTNRC6Biso1_1-1619

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1-1619 (TNRC6B Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6Biso1_1218-1723_L

Plasmid

#146850

Purpose

Insect Expression of HsTNRC6Biso1_1218-1723

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1218-1723 (TNRC6B Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6Biso1_1218-1723-F1370A_L

Plasmid

#146851

Purpose

Insect Expression of HsTNRC6Biso1_1218-1723-F1370A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1218-1723-F1370A (TNRC6B Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6Biso1-del1359-1393_L

Plasmid

#146852

Purpose

Insect Expression of HsTNRC6Biso1-del1359-1393

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1-del1359-1393 (TNRC6B Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6Biso1-del1395-1534_L

Plasmid

#146853

Purpose

Insect Expression of HsTNRC6Biso1-del1395-1534

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1-del1395-1534 (TNRC6B Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6Biso1-F1370A_L

Plasmid

#146854

Purpose

Insect Expression of HsTNRC6Biso1-F1370A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1-F1370A (TNRC6B Human)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182_862-1384-F961A_L

Plasmid

#146824

Purpose

Insect Expression of DmGW182_862-1384-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_862-1384-F961A (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-delCterm-delDUF-dsRNAres_L

Plasmid

#146827

Purpose

Insect Expression of DmGW182-delCterm-delDUF-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-delCterm-delDUF-dsRNAres (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-DUF-Cterm_L

Plasmid

#146832

Purpose

Insect Expression of DmGW182-DUF-Cterm

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-DUF-Cterm (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-DUF-RBD_L

Plasmid

#146833

Purpose

Insect Expression of DmGW182-DUF-RBD

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-DUF-RBD (gw Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-TR-del1-40

Plasmid

#180236

Purpose

Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T and R domains (r…

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R

Plasmid

#147407

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-680_D

Plasmid

#146124

Purpose

Insect Expression of DmEDC3_102-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_102-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_331-680_D

Plasmid

#146125

Purpose

Insect Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_331-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-Sirius

Plasmid

#175837

Purpose

Express Sirius with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with nucleus-targeting sequence

Tags

H2B (human histone 2B)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-KRasCT

Plasmid

#175836

Purpose

Express iRFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-Sirius

Plasmid

#174184

Purpose

Express Sirius with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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IBMc397

Plasmid

#161955

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T

Expression

Bacterial

Promoter

PBAD

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

Plasmid

#172064

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

Plasmid

#173462

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

Plasmid

#173460

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

Plasmid

#173458

Purpose

C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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