We narrowed to 12,366 results for: NUC

1. pCML 961

   Plasmid

   #98848

   Purpose

   pTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Sowa et al Nucleic Acids Res. 2017 Feb 28;45(4):167

   Inserts

   5' UTR + first 100 nucleotides of coding sequence of the aidB gene
   csrA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29a-hNABP1-C

   Plasmid

   #128311

   Purpose

   Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. NET5L pEGFP-N2 (1155)

   Plasmid

   #62035

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   NET5L (TMEM201 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   long form

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. NKP68 RFP (790)

   Plasmid

   #62003

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

   Insert

   NKP68 (TMUB1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAM-AAV-mSncg-0.66kb-EGFP

   Plasmid

   #153165

   Purpose

   Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. POPDC2 pEGFP-N2 (1087)

   Plasmid

   #62030

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   POPDC2 (POPDC2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. NET30 pEGFP-N2 (815)

   Plasmid

   #62011

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET30 (MOSPD3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLV-REPORT(EF1a)-TTN

   Plasmid

   #172327

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NET55 pEGFP-N2 (610)

   Plasmid

   #61992

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET55 (APH1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA4/TO/FLAG-hPMR1-Tev-Bio

    Plasmid

    #80125

    Purpose

    Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

    Depositor

    Daniel Schoenberg

    Article

    Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

    Insert

    PXDNL-003 (PXDNL Human)
    

    Use

    Tags

    FLAG and biotinylation

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK-SAN1-Flag

    Plasmid

    #117163

    Purpose

    Expresses Flag-tagged SAN1 nuclease in mammalian cells.

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    SAN1 (FAM120B Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM-AAV-mSncg-1.03kb-EGFP

    Plasmid

    #153164

    Purpose

    Truncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates gene expression in mammalian retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAM-AAV-hSncg-EGFP

    Plasmid

    #153198

    Purpose

    Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid

    #121834

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-Cas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

16. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid

    #121821

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. WFS1 mRFP-N2 (891)

    Plasmid

    #62018

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    WFS1 (WFS1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

    Plasmid

    #73618

    Purpose

    Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

    Plasmid

    #121828

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2Flag CMV2-ZO-2-delta-N

    Plasmid

    #27419

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Deletion of coding nucleotides from 1-351. This r…

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only