We narrowed to 12,703 results for: NUC

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pET29a-hNABP1

Plasmid

#128306

Purpose

Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChR2(H134R)-tdTomato

Plasmid

#124237

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChR2(H134R)-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAGT9050

Plasmid

#219429

Purpose

PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

AATG_PapE-4xLF2_AGGT

Use

Moclo compatible level 0 module

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HisC EXO1b

Plasmid

#68268

Purpose

Mammalian expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Tags

His

Expression

Mammalian

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Article

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Expression

Mammalian

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-2xBFP(4C)

Plasmid

#176151

Purpose

Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

NLS-2xBFP(4C)

Tags

6xHis tag and SV40 nuclear localization sequence …

Expression

Bacterial

Mutation

Compared with EGFP, the two BFP domains have the …

Promoter

Ptrc

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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Tmem38A pEGFP-N2 (1401)

Plasmid

#62050

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem38A (TMEM38A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Tmem38A mRFP-N2 (800)

Plasmid

#62007

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem38A (TMEM38A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-tdTomato-cNLS

Plasmid

#138532

Purpose

Lentiviral inducible expression of tdTomato with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

tdTomato

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE-CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NES

Plasmid

#175008

Purpose

Cytosol-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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RHBDD1 pmRFP-N2 (1390)

Plasmid

#62049

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-mCherry-cNLS

Plasmid

#138531

Purpose

Lentiviral inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE-CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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RHBDD1 pEGFP-N2 (1078)

Plasmid

#62028

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4 TNRC6A

Plasmid

#20021

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Use

Entry vector

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXa3

Plasmid

#73614

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

Chicken B-Actin

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

Plasmid

#79614

Purpose

Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Inserts

SpCas9
PmCDA1

Tags

SH3 domain and SHL

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1 and pGal10

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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