We narrowed to 2,463 results for: T2A

1. pJW1511

   Plasmid

   #62365

   Purpose

   pEF1a-puro-T2A-ATHmmuRPL10A

   Depositor

   Jonathan Weissman

   Article

   Jan et al Science. 2014 Nov 7;346(6210):1257521. d

   Insert

   Puro-T2A-AVITEVHAmmuRPL10A
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

   Plasmid

   #204722

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZHY013

   Plasmid

   #36185

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Insert

   Heterodimeric FokI nuclease
   

   Use

   Entry vector for expression in plants as a t2a li…

   Promoter

   none

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYL116

   Plasmid

   #239967

   Purpose

   CRISPR donor plasmid with Puro-T2A-mCherry cassette for Suz12 disruption

   Depositor

   Thomas Cech

   Article

   Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

   Insert

   PuroR-T2A-mCherry with homology arms for human Suz12
   

   Use

   CRISPR

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYL117

   Plasmid

   #239968

   Purpose

   CRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption

   Depositor

   Thomas Cech

   Article

   Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

   Insert

   PuroR-T2A-mCherry with homology arms for human Ezh2
   

   Use

   CRISPR

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

   Plasmid

   #204729

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

   Plasmid

   #204727

   Purpose

   Frt-inducible CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

   Plasmid

   #204724

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRM209 LTP (Luciferase tdTomato Puro)

   Plasmid

   #174723

   Purpose

   Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   tdTomato
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and T2A linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-DNMT3A-PuroR_hNT

    Plasmid

    #71830

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    Non-targeting sgRNA human (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPL001

    Plasmid

    #184750

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-Cas9-Blast

    Plasmid

    #83481

    Purpose

    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

    Depositor

    Mohan Babu

    Inserts

    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    Tight Tre and human phosphoglycerate kinase 1 pro…

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJA291

    Plasmid

    #74487

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    puroR-mCherry-T2A-eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHACK-QF2-AgU6-mCherry

    Plasmid

    #184530

    Purpose

    HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

    Depositor

    Christopher Potter

    Article

    Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

    Insert

    QF2
    

    Use

    CRISPR

    Promoter

    Anopheles U6 promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TO-hNGN2-BSD-mApple

    Plasmid

    #182308

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    hNGN2 (NEUROG2 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ac5-STABLE2-neo

    Plasmid

    #32426

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    Flag-mCherry-T2A-GFP-T2A-neo
    

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDT305

    Plasmid

    #128801

    Purpose

    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    

    Use

    CRISPR and Lentiviral

    Promoter

    hUBC promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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