We narrowed to 2,526 results for: T2A
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Plasmid#234711PurposepAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linkerDepositorInsertmKate2.0 T2A N2cG
UseAAVTagsT2AExpressionMammalianPromoterCAGAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2642
Plasmid#229526PurposeCarries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2643
Plasmid#229527PurposeCarries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mAID_optExpressionBacterial and YeastMutationF74GAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TC374
Plasmid#122614PurposePrpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
ExpressionWormAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TC476
Plasmid#122629PurposePunc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
ExpressionWormAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TC393
Plasmid#122625PurposePser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertrtetR-QFAD::P2A::mKate::T2A::tetR-pie1
ExpressionWormAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2647
Plasmid#229989PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2649
Plasmid#229991PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP (ANV)
Plasmid#71685PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_v2
Plasmid#74407PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)
Plasmid#71684PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.3
Plasmid#190797Purposeaka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final productDepositorInsert2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Split PS Intein
Plasmid#242022PurposeBlue-light-induced reconstitution of split mCherry. A T2A peptide is inserted at the natural split site of gp41-1.DepositorInsertSplit PS Intein
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9
Plasmid#182310PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only