We narrowed to 3,093 results for: sam

1. pLKO-Cre stuffer v3

   Plasmid

   #158030

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pRK5-HA-metap2

   Plasmid

   #100512

   Purpose

   Expresses HA tagged metap2 in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH68 (dAsCas12a-3xKRAB piggyBac)

   Plasmid

   #217334

   Purpose

   piggyBac expression of dAsCas12a-3xKRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   dAsCas12a
   

   Use

   CRISPR

   Tags

   nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3 FLAG_CMV14/OCTN2 (wt)

   Plasmid

   #112799

   Purpose

   Expression of OCTN2

   Depositor

   Katarzyna Nalęcz

   Article

   Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

   Insert

   OCTN2/SLC22A5 (Slc22a5 Rat)
   

   Tags

   3 x Flag

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEY14

   Plasmid

   #191059

   Purpose

   acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-5(orf) (acr-5 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-STAG2-wildtype

   Plasmid

   #31972

   Depositor

   Todd Waldman

   Article

   Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

   Insert

   human STAG2 cDNA (STAG2 Human)
   

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-hSOX10

   Plasmid

   #24749

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Mutation

   wildtype sequence

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

8. LVDP 2P.2

   Plasmid

   #231904

   Purpose

   For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
   

   Use

   Lentiviral

   Mutation

   R203M in N protein

   Promoter

   CMV, EF-1a

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-hs-3xHA-LIN-41

   Plasmid

   #52717

   Purpose

   retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-hs-LIN-41

    Plasmid

    #52716

    Purpose

    retroviral expression of human LIN-41 / TRIM71

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-ABKAR

    Plasmid

    #61508

    Purpose

    Expression of AMPK biosensor targeted to the endoplasmic reticulum

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Tags

    ER targeting sequence from CYP450 2C1 (see commen…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBa.Halo-myosin Va tail 1006-1828

    Plasmid

    #162715

    Purpose

    Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Va (Myo5a Rat)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1005 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-CBS (WT)

    Plasmid

    #182923

    Purpose

    Mammalian expression plasmid for human CBS

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PM-ABKAR

    Plasmid

    #61506

    Purpose

    Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Tags

    Lyn kinase sequence (see comments)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pU6-DHFR

    Plasmid

    #80329

    Purpose

    Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    DHFR-TSc3
    

    Use

    CRISPR

    Promoter

    DHFR-TS 5' UTR

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBa.GFP-myosin Vb tail 1436-1846

    Plasmid

    #162714

    Purpose

    Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Vb (Myo5b Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1435 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEY139

    Plasmid

    #223486

    Purpose

    tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    tph-1 (tph-1 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEY82

    Plasmid

    #191077

    Purpose

    flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-7 (flp-7 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX-mTAF6

    Plasmid

    #44337

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF6
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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