We narrowed to 2,735 results for: ubiquitin

Showing: 1501 - 1520 of 2735 results

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pGFP-mTet1-DSBH pc2335

Plasmid

#201697

Purpose

Expresses of GFP-tagged mTet1-DSBH in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 DSBH (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-delCys pc3903

Plasmid

#201701

Purpose

Expresses of GFP-tagged mTet1-delCys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the Cysteine rich domain of Tet1

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s-delCys pc3904

Plasmid

#201702

Purpose

Expresses of GFP-tagged mTet1s-delCys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the Cysteine rich domain of Tet1s

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-D3-ASK1

Plasmid

#226619

Purpose

Co-expression of D3 and ASK1 complex in insect cells

Depositor

Ning Zheng

Article

Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

Inserts

D3
ASK1 (SKP1 Mustard Weed)

Tags

His tag followed with three copies of Msyb

Expression

Insect

Mutation

The flexible loop in D3 (Q478-D515) can be remove…

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852R pc3914

Plasmid

#201704

Purpose

Expresses of GFP-tagged mTet1s_K852R in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852R (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to arginine

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s_K852E pc3915

Plasmid

#201705

Purpose

Expresses of GFP-tagged mTet1s_K852E in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s-K852E (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 852 mutated to glutamate

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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rUBR5_2383-2442_pGEX6P1

Plasmid

#209791

Purpose

Bacterial expression of UBR5 Mlle domain

Depositor

Kalle Gehring

Article

Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

Insert

Mlle domain of UBR5 (Ubr5 Rat)

Tags

GST

Expression

Bacterial

Mutation

aa 2383-2442 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-NDP52

Plasmid

#190194

Purpose

Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b p97-His D592Amb

Plasmid

#169022

Purpose

Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His

Expression

Bacterial

Mutation

D592TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 S1213 P1214R

Plasmid

#166830

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Mutation

P1214R

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 L1215

Plasmid

#166828

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 S1213

Plasmid

#166827

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 L1218

Plasmid

#166829

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBW_0005

Plasmid

#102837

Purpose

Binary vector containing Sr22 PI573523 allele driven by maize ubiquitin promoter, Sr22 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 PI573523 (NEWENTRY Synthetic)

Expression

Plant

Promoter

Maize ubiquitin

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE Y165A

Plasmid

#139428

Purpose

GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed tyrosine 165 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE P199A

Plasmid

#139427

Purpose

GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed proline 199 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7RE I137A

Plasmid

#139319

Purpose

GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed isoleucine 137 to an alanine

Promoter

T7 promoter-1

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B Q116P G120

Plasmid

#129291

Purpose

Gateway entry clone encoding human deconjugation-resistant LC3B

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Mutation

Changed Glutamine 116 to Proline. Deleted amino a…

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [Y252D]

Plasmid

#126550

Purpose

Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Y252D point mutant

Promoter

LTR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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