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We narrowed to 4,416 results for: BRE

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Showing: 3801 - 3850 of 4416 results

  10. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  11. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  12. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  13. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  20. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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  22. SDC4A-c001

    Plasmid
    #205119
    Purpose
    SDC4 protein expression
    Depositor
    Paul Brennan
    Insert
    SDC4 (SDC4 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205119-plasmid-map-sequence-id-411015

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  23. QPRTA-c002

    Plasmid
    #205115
    Purpose
    QPRT protein expression
    Depositor
    Paul Brennan
    Insert
    QPRT (QPRT Human)
    Tags
    TEV-6His
    Expression
    Insect
    Mutation
    WT
    Available Since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205115-plasmid-map-sequence-id-410409

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  24. pOpen-HSlig4

    Plasmid
    #165503
    Purpose
    Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase IV
    Use
    Synthetic Biology
    Available Since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165503-plasmid-map-sequence-id-338457

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  25. SLIT2A-c003

    Plasmid
    #205125
    Purpose
    SLIT2 protein expression
    Depositor
    Paul Brennan
    Insert
    SLIT2 (SLIT2 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205125-plasmid-map-sequence-id-412271

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  26. SLIT1A-c003

    Plasmid
    #205124
    Purpose
    SLIT1 protein expression
    Depositor
    Paul Brennan
    Insert
    SLIT1 (SLIT1 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205124-plasmid-map-sequence-id-412475

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  27. SDCBPA-c023

    Plasmid
    #205121
    Purpose
    SDCBP protein expression
    Depositor
    Paul Brennan
    Insert
    SDCBP (SDCBP Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205121-plasmid-map-sequence-id-411056

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  28. pLenti6.3_HES4

    Plasmid
    #124072
    Purpose
    HES4 with normal stop codon
    Depositor
    J. Chad Brenner
    Insert
    HES4 (HES4 Human)
    Use
    Lentiviral
    Available Since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124072-plasmid-map-sequence-id-241438

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  29. SFRP1A-c500

    Plasmid
    #205122
    Purpose
    SFRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    SFRP1 (SFRP1 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205122-plasmid-map-sequence-id-412368

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  30. SFRP1A-c501

    Plasmid
    #205123
    Purpose
    SFRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    SFRP1 (SFRP1 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205123-plasmid-map-sequence-id-412370

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  31. TMEFF2A-c005

    Plasmid
    #205127
    Purpose
    TMEFF2 protein expression
    Depositor
    Paul Brennan
    Insert
    TMEFF2 (TMEFF2 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205127-plasmid-map-sequence-id-412277

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  32. TMEFF2A-c004

    Plasmid
    #205126
    Purpose
    TMEFF2 protein expression
    Depositor
    Paul Brennan
    Insert
    TMEFF2 (TMEFF2 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205126-plasmid-map-sequence-id-412272

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  33. SDCBPA-c012

    Plasmid
    #205120
    Purpose
    SDCBP protein expression
    Depositor
    Paul Brennan
    Insert
    SDCBP (SDCBP Human)
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205120-plasmid-map-sequence-id-411022

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  38. PTNA-c200

    Plasmid
    #205114
    Purpose
    PTN protein expression
    Depositor
    Paul Brennan
    Insert
    PTN (PTN Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205114-plasmid-map-sequence-id-411200

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  40. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

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  41. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

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  42. pNSU299_SEO1

    Plasmid
    #166076
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SEO1 (SEO1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166076-plasmid-map-sequence-id-329607

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  45. pLenti6.3_HES2

    Plasmid
    #124070
    Purpose
    HES2 with normal stop codon
    Depositor
    J. Chad Brenner
    Insert
    HES2 (HES2 Human)
    Use
    Lentiviral
    Available Since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    124070-plasmid-map-sequence-id-244596

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  46. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

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  47. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  48. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  49. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

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  50. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

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Showing: 3801 - 3850 of 4416 results