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Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d80
Plasmid#114030PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S15D
Plasmid#114031PurposeInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell linesDepositorInserteGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 8A/S15DPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
hsp70:EB3-mKate2
Plasmid#105965Purposeheat shock inducible transgenesisDepositorInsertEB3 (MAPRE3 Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5A1-T
Plasmid#92017PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD2024
Plasmid#101368PurposeName: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).DepositorInsertRBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseTagsExpressionMutationRBPjk binding site A mutation in pCBG68-LANApi (p…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2023
Plasmid#101367PurposeName: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site B mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBR-K14 (pDD200…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2028
Plasmid#101371PurposeName: pCBR-RBPjkΔA. RBPjk binding site A mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site A mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationRBPjk binding site A mutation in pCBR-K14 (pDD200…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2030
Plasmid#101373PurposeName: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationRBPjk binding site B mutation in pBluescript-LANA…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2031
Plasmid#101374PurposeName: pBluescript-RBPjkΔA. RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationRBPjk binding site A mutation in pBluescript-LANA…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5C3-T
Plasmid#92022PurposeExpresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting sucA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 N184X
Plasmid#92373PurposeInducible expression of M87 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, N184XPromoterTet-responsive P tightAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP S245X with 5'UTR
Plasmid#92376PurposeInducible expression of S245X spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationS245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87 S245X
Plasmid#92374PurposeInducible expression of M87 S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, S245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 S245X
Plasmid#92372PurposeInducible expression of M1S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, S245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 N184X
Plasmid#92371PurposeInducible expression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mPar6a K19A
Plasmid#86149PurposeFLAG-mPar6a K19A - Does not bind to aPKCDepositorInsertPar6 (Pard6a Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationLysine to Alanine mutation K19APromoterLTRAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_muBim_Ex3
Plasmid#85533PurposeInducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporterDepositorInsertmu Bim
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-WTSI
Plasmid#80486PurposepiggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)DepositorInsertWTSI
UsePiggybac transposonTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch5_155183064-155183141
Plasmid#81211PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA5_no stop
Plasmid#83868PurposeGateway donor vector for IAA5 with no stop codonDepositorInsertIAA5 (IAA5 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA4_no stop
Plasmid#83866PurposeGateway donor vector for IAA4 with no stop codonDepositorInsertIAA4 (ATAUX2-11 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 DM
Plasmid#61931PurposeExpression of hUCH37 (ISF1) M269E/N291D in E.coliDepositorInserthUCH37 (ISF1) M269E/N291D (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationChanged methionine 269 to glutamate and asparagin…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148A/F149A)
Plasmid#61933PurposeExpression of hUCH37 (M148A/F149A) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationMethionine 148 and Phenylalanine 149 were mutated…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW094
Plasmid#64757PurposeRecombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-6xHIS epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT005
Plasmid#64760PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an HA-6xHIS epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_K36E_R38E
Plasmid#65095PurposeDestination vector with RBPMS with K36E/R38E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange lysine 36 to glutamic acid and arginine 38…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38Q
Plasmid#65094PurposeDestination vector with RBPMS with R38Q mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged arginine 38 to glutaminePromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1853
Plasmid#53040PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st2), RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJC25R1+4stop
Plasmid#17723DepositorInsertSup35R1+4 (SUP35 Budding Yeast)
UseTagsExpressionBacterialMutationE.coli expression plasmid for NM-region of Sup35 …PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG72QDProCFPp303
Plasmid#15590DepositorInserthtt 72Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B V304A,I367A IRES GFP
Plasmid#133821PurposeEncodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationV304A, I367APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B D363,365N IRES GFP
Plasmid#133822PurposeEncodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationD363,365NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A K189-192A IRES GFP
Plasmid#133823PurposeEncodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationK189-192APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A D230,232N IRES GFP
Plasmid#133824PurposeEncodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationD230,232NPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A M173,F234A IRES GFP
Plasmid#133825PurposeEncodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationM173, F234APromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDD2022
Plasmid#101366PurposeName: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).DepositorInsertRBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBG68-LANApi (p…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLN428 (FuGW-G5p-mKate2-1Pe )
Plasmid#105184PurposeModule 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG5p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLN434 (FuGW-G14p-mKate2-1Pe )
Plasmid#105188PurposeModule 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG14p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
UseTagsExpressionMammalianMutationPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
UseTagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_H2A_C1
Plasmid#85053PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex5
Plasmid#85535PurposeInducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporterDepositorInsertmu p53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-1P-Kv-WPRE
Plasmid#202612PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P-Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only