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His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
UseTags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
UseTags(-30)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SIMx6
Plasmid#116938PurposeBacterial expression vector for 6 simDepositorInsert6 sim
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SUMOx10
Plasmid#116937PurposeBacterial expression vector for 10 sumoDepositorInsert10 SUMO
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
drebrin-His
Plasmid#40363DepositorInsertdrebrin E2 (DBN1 Human)
UseTagsHis and T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneUseTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialMutationPromoterT7-LacOAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pHsSR beta deltaN Flag
Plasmid#13731DepositorInsertSignal recognition particle receptor β subunit (SRPRB Human)
UseTags6xHis and FLAGExpressionBacterialMutationexpresses residues 59-271 of Homo sapiens SRβ wit…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKD077
Plasmid#136473PurposeSSB-mTur2 IDL fusion expression plasmidDepositorInsertSSB-mTur2 (ssb E. coli)
UseTagsmTurquoise2ExpressionBacterialMutationmTurquoise2 inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
UseTagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHYP-NPC-10H
Plasmid#162789PurposeSARS-CoV-2 Nucleocapsid Protein expression in E.coliDepositorInsertNucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
UseTags10xHisExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP3 CKK
Plasmid#59033Purposeexpresses CKK domain of CAMSAP3 in E. coliDepositorInsertCAMSAP3 CKK domain (CAMSAP3 Mouse)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-SUMO-RNF168ring (1-189)
Plasmid#63570PurposeExpression of human RNF168 ring domain (residues 1-189) tagged with His-SUMODepositorInsertRNF168 (RNF168 Human)
UseTags6xHis and SUMO3ExpressionBacterialMutationRing domain only (residues 1-189)PromoterT7Available sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CFP-FRBx5-His
Plasmid#103784Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertCFP-FRBx5-His
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5-His
Plasmid#103785Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertYFP-FKBPx5-His
UseTagsEYFP, His-tagExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP2 CKK
Plasmid#59032Purposeexpresses CKK domain of CAMSAP2 in E. coliDepositorInsertCAMSAP2 CKK domain (CAMSAP2 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZ005 (AacC2c1 human codon optimized protein)
Plasmid#70171PurposeExpresses AacC2c1 human codon optimized proteinDepositorInsertAacC2c1 protein
UseTagsNLSExpressionMutationPromoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP1 CKK
Plasmid#59031Purposeexpresses CKK domain of CAMSAP1 in E. coliDepositorInsertCAMSAP1 CKK domain (CAMSAP1 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMtb CblA
Plasmid#138298PurposeExpression of Mtb CblADepositorInsertMtb CblA
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMtb ATR
Plasmid#138297PurposeExpression of Mtb ATRDepositorInsertCorrinoid adenosyl transferase
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHis-hPim1
Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
UseTagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GRLF1
Plasmid#36195PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains the Ras homolog domain only (amino acid …PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
HpMscS
Plasmid#78310PurposeH. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG TagDepositorInsertMechanosensitive channel of small conductance
UseTags6x His Tag and FLAG TagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ N238A
Plasmid#129648PurposeExpresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequenceDepositorInsertmetQ Gene
UseTags10x Histidine Tag, Enterokinase, and pelB signal …ExpressionBacterialMutationChanged Asparagine 238 to AlaninePromoterT7 PromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP8
Plasmid#135553PurposeExpress mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP8
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p28atDarpp
Plasmid#108318PurposeExpresses tDarpp in E.coliDepositorInserttDarpp (PPP1R1B Human)
UseTags6 HistadineExpressionBacterialMutationPromoterT7Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-ZZ(1700-1751)
Plasmid#99334PurposeBacterial expression of mouse CBP ZZ domainDepositorInsertCBP ZZ domain (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZ004 (LseC2c2 locus)
Plasmid#70170PurposeExpresses LseC2c2 locus with one spacer in arrayDepositorInsertLseC2c2 locus with one spacer in array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
OphA-delta C6
Plasmid#127679PurposeBacterial expression of His-tagged OphA-delta C6DepositorInsertOphA-delta C6
UseTagsHis8ExpressionBacterialMutationdelta C6PromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
MolBC
Plasmid#92210PurposeExpression of H. influenzae MolBC (ABC Transporter) in E. coliDepositorUseTags8x Histidine Tag and Enterokinase Cleavage Site b…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13i, C77A, MG-31-28)
Plasmid#110747PurposeBacterial expression of human USP30 (construct 13i)DepositorInsertUSP30 (USP30 Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationC77A, F348D, M350S, I353E + insertion deletion: 6…PromoterT7Available sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-NEMOL329P
Plasmid#11969DepositorInsertNEMOL329P (IKBKG Human)
UseTagsHisExpressionBacterialMutationLeucine329 to ProlinePromoterAvailable sinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXD13 (chicken) HIS-tag pET
Plasmid#8555DepositorInsertHOXD13 (HOXD13 )
UseTagsHis and T7ExpressionBacterialMutationcontains 10 nt 5' to ATG start; makes prote…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
Dom34(265-386)
Plasmid#19102DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tag and N tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34(265-377)
Plasmid#19103DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tag and N tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Human Mevalonate diphosphate decarboxylase (MDD)
Plasmid#52431Purposebacterial expression of human Mevalonate diphosphate decarboxylase (MDD)DepositorInsertmevalonate diphosphate decarboxylase (MVD Human)
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIIS793
Plasmid#238206PurposeExpresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanRDepositorInsertTaTasR
UseTagsTwinStrepII-bdSUMOExpressionBacterialMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
NLuc-His10
Plasmid#234567PurposeBacterially expressed NLuc for Ni-NTA purificationDepositorInsertNano luciferase
UseTagsT7 gene 10 leader peptide and Thrombin site-10xHi…ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterT7Available sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
NP-11-63
Plasmid#234358PurposeExpressing and purifying R.rubrum rubisco using a his-sumo tagDepositorInsertR.rubrum rubisco
UseSynthetic BiologyTagsN-term SUMO tagExpressionMutationPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only
