Addgene

We narrowed to 38,357 results for: LAT;

Showing: 1521 - 1540 of 38357 results

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L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
SEPT5 WT

Plasmid

#27272

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER.PKD3.RNAi

Plasmid

#10818

Depositor

Alex Toker

Article

Hausser et al Nat Cell Biol. 2005 Sep . 7(9):880-6.

Insert

PKD3 RNAi (PRKD3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCMV5-HA-mTmod3-WT

Plasmid

#70752

Purpose

N-terminal-HA-tagged mouse Tropomodulin3, WT

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

Plasmid

#58380

Purpose

tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

avi-EGFP-rpl10a

Use

Tags

Avi Tag and EGFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Use

Tags

GST and His6

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pWH-DI

Plasmid

#47385

Purpose

Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

dxs
idi

Use

Tags

Expression

Bacterial

Mutation

Promoter

PxylA

Available since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only
vYM014_phiC31-nTRE-Ins(low)-pEF1s(low)-H2b-Citrine

Plasmid

#197682

Purpose

"Insert(low)-pEF1s(low)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Promoter

Available since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Dlx2

Plasmid

#15537

Depositor

John Rubenstein

Article

Porteus et al Neuron. 1991 Aug . 7(2):221-9.

Insert

Dlx2 (Dlx2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Partial cDNA

Promoter

Available since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pMDC32-SEIPIN1

Plasmid

#96973

Purpose

Express Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLIA-E p27 (CC#620)

Plasmid

#15191

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Use

Tags

FLAG and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK0

Plasmid

#45047

Purpose

FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S

Plasmid

#149638

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, empty

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Use

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only