We narrowed to 3,633 results for: XIA

1. pcDNA3 FlipGFP(Mpro) T2A mCherry

   Plasmid

   #163078

   Purpose

   Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

   Depositor

   Xiaokun Shu

   Article

   Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

   Insert

   FlipGFP-based Mpro activity reporter and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4-NIK-HA

   Plasmid

   #27554

   Depositor

   Shao-Cong Sun

   Article

   Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

   Insert

   NIK (MAP3K14 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   NIK starts at amino acid 3; C52R and R139H mutati…

   Promoter

   CMV

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pEBG-Pak1 83-149

   Plasmid

   #12214

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Dominant negative Pak1: autoinhibitory domain, aa…

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Merlin S518A

   Plasmid

   #84294

   Purpose

   Expression of Merlin with an alanine mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518A

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Merlin S518D

   Plasmid

   #84295

   Purpose

   Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518D

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS4134

   Plasmid

   #131747

   Purpose

   Human reduced folate transporter 1 SLC19A1 for lentiviral transduction, copGFP transduction marker

   Depositor

   Christina Smolke

   Article

   Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

   Insert

   Human reduced folate carrier 1 (SLC19A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. C204

   Plasmid

   #174358

   Purpose

   For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (PABPC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Multiple silent mutations introduced to be resist…

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-T5-FB-E25TAG-F6C

   Plasmid

   #176546

   Purpose

   Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change phenylalanine 6 to cysteine; change glutam…

   Promoter

   T5

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBG-Pak1 83-149 L107F

   Plasmid

   #12215

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Autoinhibitory domain (aa 83-149) with L107F muta…

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCMV4-p100SS/AA (SS866/870AA)

    Plasmid

    #27555

    Depositor

    Shao-Cong Sun

    Article

    Xiao et al EMBO J. 2001 Dec 3. 20(23):6805-15.

    Insert

    p100 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serines 866 and 870 mutated to Alanines

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

11. ABE-RA5.5

    Plasmid

    #208316

    Purpose

    Expresses ABE-RA5.5 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA5.5-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXY029 (mEos2-MTSBs)

    Plasmid

    #72652

    Purpose

    Inducible expression of mEos2-MTSBs in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    mEos2-MTSBs
    

    Use

    Tags

    mEos2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-HIF2α wt-pBabe-Puro

    Plasmid

    #26055

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205316

    Purpose

    All-in-one AAV vector expressing ISAam1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAam1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-EGFP

    Plasmid

    #209057

    Purpose

    Gateway-compatible entry vector, with insert of the EGFP gene

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205317

    Purpose

    All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISYmu1 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205318

    Purpose

    All-in-one AAV vector expressing ISDge10 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISDge10 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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