We narrowed to 4,160 results for: biorxiv

1. dSp-p65 (150-261)

   Plasmid

   #99655

   Purpose

   Expresses dSp Cas9 fused to truncated p65 activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated p65 activation domain (150-261)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. dSp-p65 (100-261)

   Plasmid

   #99656

   Purpose

   Expresses dSp Cas9 fused to truncated p65 activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated p65 activation domain (100-261)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. dSp-p65 (200-261)

   Plasmid

   #99657

   Purpose

   Expresses dSp Cas9 fused to truncated p65 activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated p65 activation domain (200-261)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. dSp-p65 (1-200)

   Plasmid

   #99658

   Purpose

   Expresses dSp Cas9 fused to truncated p65 activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated p65 activation domain (1-200)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dSp-p65 (1-150)

   Plasmid

   #99659

   Purpose

   Expresses dSp Cas9 fused to truncated p65 activation domain

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   truncated p65 activation domain (1-150)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MBP-3C-hTspan12LEL(118-218)_pAcGP67a

   Plasmid

   #216383

   Purpose

   Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Tetraspanin-12 (TSPAN12 Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

   Plasmid

   #218654

   Purpose

   Knockout vector for mouse Fam160b1 (Fhip2A)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Fam160b1 (Fhip2a Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

   Plasmid

   #218655

   Purpose

   Knockout vector for mouse Fam160b1 (Fhip2A)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Fam160b1 (Fhip2a Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

   Plasmid

   #218636

   Purpose

   Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   H781E, Q782R, F785A

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

    Plasmid

    #218635

    Purpose

    Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    N1008R, R1009E, E1010R, R1013E

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GOLIM4 g2 LentiCRISPRv2-mCherry

    Plasmid

    #218663

    Purpose

    Knockout vector for human GOLIM4 (GPP130/GOLPH4)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    GOLIM4 (GOLIM4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GOLIM4 g1 LentiCRISPRv2-mCherry

    Plasmid

    #218662

    Purpose

    Knockout vector for human GOLIM4 (GPP130/GOLPH4)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    GOLIM4 (GOLIM4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-SFFVp-gI-BlastR

    Plasmid

    #207179

    Purpose

    Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFVp

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SV40p-gI-BlastR

    Plasmid

    #207182

    Purpose

    Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1_NAP1-eGFP

    Plasmid

    #216837

    Purpose

    For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Efg1-ΔDBD-Q443P

    Plasmid

    #216401

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Glutamine 443 to Proline, and deleted ami…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

    Plasmid

    #217725

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE4
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-179

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

    Plasmid

    #215000

    Purpose

    Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-6xHis-TrxF

    Plasmid

    #207454

    Purpose

    E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

    Depositor

    Sarah Stainbrook, Joseph Jez

    Article

    Stainbrook et al bioRxiv 2022.08.24.505184

    Insert

    Thioredoxin F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    T7

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210749

    Purpose

    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    CjSp D8A Cas9
    Bilbo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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