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pCSm5En2CER4(#457)

Plasmid

#184090

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2(C>S)-ERT2

Use

Synthetic Biology

Tags

5xMyc

Expression

Mammalian

Mutation

chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…

Promoter

sCMV+SP6

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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QuasAr-P2A-hKvb1

Plasmid

#154083

Purpose

Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

Lentiviral

Tags

myc-Flag

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-L2

Plasmid

#122307

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1 (Hdac1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152, R42W)

Plasmid

#110767

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

R42W

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-eGFP-3HA

Plasmid

#108393

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4077-3HA

Plasmid

#108392

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4077-eGFP

Plasmid

#108391

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

Plasmid

#224435

Purpose

AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mScarlet-KASH
U6-gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#210525

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Use

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

Plasmid

#210523

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

Plasmid

#213481

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-APEX2-Ires-eGFP-PAC

Plasmid

#180142

Purpose

used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB1

Plasmid

#120361

Purpose

Produce human recombinant TGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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