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We narrowed to 9,949 results for: Coli

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Showing: 7731 - 7740 of 9949 results

  6. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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  8. pHIs8-4b-SaDAH::G226D

    Plasmid
    #140130
    Purpose
    Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli
    Depositor
    Jing-Ke Weng
    Article
    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:
    Insert
    Dechloroacutumine halogenase G226D variant from Sinomenium acutum
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    changed Gly226 to Asp226
    Promoter
    T7
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140130-plasmid-map-sequence-id-275079

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Showing: 7731 - 7740 of 9949 results