We narrowed to 9,789 results for: Coli
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Plasmid#101166PurposeE. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4-V5-His6
Plasmid#67419PurposeExpression of hArl4 with C-term V5 and His6 tag in E. coliDepositorInsertARL4 (ARL4A Human)
TagsHis and V5ExpressionBacterialMutationbp 105 A to T; silent mutationPromoterT7/lacOAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSTTa-GPA1
Plasmid#248648PurposeVector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1 in E. coli.DepositorInsertGPA1 (GP ALPHA 1 Mustard Weed)
Tags2x StrepII, Thrombin site, TEV siteExpressionBacterialPromotertacAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSTTa-GNAO1
Plasmid#248652PurposeVector for protein expression of 2x StrepII-tagged human heterotrimeric G protein GNAO1 in E. coli.DepositorInsertGNAO1 (GNAO1 Human)
Tags2x StrepII, Thrombin site, TEV siteExpressionBacterialPromotertacAvailable SinceJan. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAIDA_TEV_fap2EC
Plasmid#246866PurposeExpression of Fusobacterium nucleatum ATCC23726 Fap2 extracellular domain (42-3271) with histag and TEV site in fusion with E. coli autotransporter AIDADepositorInsertF. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)
Tags8x histidine, AIDA autotransporter, AIDA signal s…ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-p120RasGAP-714-1047-WT
Plasmid#245307PurposeExpresses GAP domain of human p120RasGAP (residues 714-1047 wild type) in E. coliDepositorInsertRASA1 (RASA1 Human)
Tags6HisExpressionBacterialMutationdeleted amino acids 1-713PromoterT7lacAvailable SinceOct. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-p120RasGAP-581-1047-WT
Plasmid#246487PurposeExpresses C2 and GAP domains of human p120RasGAP (residues 581-1047 wild type) in E. coliDepositorInsertRASA1 (RASA1 Human)
Tags6HisExpressionBacterialMutationdeleted amino acids 1-580PromoterT7lacAvailable SinceOct. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS2096
Plasmid#239835PurposeNovaIscB E. coli expression for recombinant purificationDepositorInsertNovaIscB
Tags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationInsertion of REC domain from NbaCas9-1 and swap o…PromoterT7Available SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-CDA1
Plasmid#229535PurposepMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsdnCas3
Cytidine deaminase
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-APOBEC1
Plasmid#229537PurposepMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
dnCas3
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only