165,960 results

1. VPS13D^EGFP

   Plasmid

   #174109

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   With an internal EGFP tag in aminoacid position 1…

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-tdTomato

   Plasmid

   #83029

   Purpose

   expresses tdTomato fluorescent protein under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

   Insert

   tdTomato
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer20

   Plasmid

   #44012

   Purpose

   Tet-inducible lentiviral vector for ORF expression

   Depositor

   Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

   Plasmid

   #190163

   Purpose

   Microglia-targeted transgene expression.

   Depositor

   Hirokazu Hirai

   Article

   Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

   Inserts

   mIba1 promoter, 1.7-kb, microglia-specific promoter
   miR-9-Tx4, 4 repeated miRNA target sites for miR-9
   miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
   

   Use

   AAV

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

   Plasmid

   #188777

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMKO.1 GFP

   Plasmid

   #10676

   Purpose

   Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. LMAN2-bio-His

   Plasmid

   #51923

   Purpose

   Expresses full-length Vesicular integral-membrane protein VIP36 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LMAN2 (LMAN2 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC37A4

   Plasmid

   #131914

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC37A4 (SLC37A4 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. HSD11B1

   Plasmid

   #39155

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSD11B1 (HSD11B1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   T220I; codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pEVS104

    Plasmid

    #207393

    Purpose

    Conjugal helper plasmid

    Depositor

    Jason Peters, Edward Ruby, Eric Stabb

    Article

    Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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