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Showing: 1541 - 1560 of 4689 results

  1. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid
    #236245
    Purpose
    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mTagBFP2
    Expression
    Mutation
    Promoter
    U6 and human Synapsin1
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236245-plasmid-map-sequence-id-471239

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  2. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

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  3. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

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  4. OgeuIscB CTG RNA

    Plasmid
    #222865
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a CTG target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (CTG)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 17, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222865-plasmid-map-sequence-id-449444

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  5. OgeuIscB GCT RNA

    Plasmid
    #222862
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a GCT target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (GCT)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222862-plasmid-map-sequence-id-449454

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  6. OgeuIscB GCA RNA

    Plasmid
    #222864
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a GCA target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (GCA)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222864-plasmid-map-sequence-id-449452

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  7. OgeuIscB TGC RNA

    Plasmid
    #222863
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a TGC target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (TGC)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222863-plasmid-map-sequence-id-449446

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  8. OgeuIscB AGC RNA

    Plasmid
    #222866
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a AGC target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (AGC)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222866-plasmid-map-sequence-id-449445

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  9. OgeuIscB CAG RNA

    Plasmid
    #222867
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a CAG target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (CAG)6 target sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222867-plasmid-map-sequence-id-449443

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  10. pLKO.1 LARP7-FLAG 7SK

    Plasmid
    #200733
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    200733-plasmid-map-sequence-id-401803

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  11. pX330.orup

    Plasmid
    #110404
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110404-plasmid-map-sequence-id-307700

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  12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  13. pDest_U6_A2_CG2_T1snp_gRNA

    Plasmid
    #213789
    Purpose
    pDEL114; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I (SNP) sgRNA
    Depositor
    William Talbot
    Insert
    nrg1 type I SNP sgRNA (nrg1 Zebrafish)
    Use
    Tol2 destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    213789-plasmid-map-sequence-id-421038

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  14. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6-3
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

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  19. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113194-plasmid-map-sequence-id-216314

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  20. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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Showing: 1541 - 1560 of 4689 results