We narrowed to 6,346 results for: rel

1. eSpCas9-EF-5Nanog_2

   Plasmid

   #174871

   Purpose

   CRISPR vector for generating Nanog STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Nanog (Nanog Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSMT3_SRSF1

   Plasmid

   #201056

   Purpose

   Express sumo-tagged human SRSF1

   Depositor

   Jun Zhang

   Article

   Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

   Insert

   SRSF1 (SRSF1 Human)
   

   Use

   Tags

   sumo

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SBC015866

   Plasmid

   #226280

   Purpose

   Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

   Depositor

   Rainer Breitling

   Article

   Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

   Inserts

   4'-phosphopantetheinyl transferase Sfp
   Cinnamyl alcohol dehydrogenase
   Carboxylic acid reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCDNA5 TEX264wt-cSSH

   Plasmid

   #220209

   Purpose

   Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Strep-strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 Zscan4 promoter

   Plasmid

   #69249

   Purpose

   Luciferase reporter for Dux4 activation of Zscan4 transcription

   Depositor

   Stephen Tapscott

   Article

   Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

   Insert

   Zscan4 (ZSCAN4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   The two single nucleotide mismatches found betwee…

   Promoter

   Zscan4

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

   Plasmid

   #132685

   Purpose

   AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Carnosine dipeptidase 2 (Cndp2 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSynapsin_NLS_GFP_P2A_SNAP_NR1

   Plasmid

   #170458

   Purpose

   Expresses SNAP tagged NR1 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   NR1 (Grin1 Rat)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHEN-CoV2-nanobody-E2

   Plasmid

   #194590

   Purpose

   Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   E2 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PH-Btk(R28C)-GFP

   Plasmid

   #51464

   Purpose

   Non-PIP3 binding control for PH-Btk-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R28C

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

10. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EFS-DIO-GRAB_Ado1.0med

    Plasmid

    #153292

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHiUGE smFP-V5 donor (all-in-one) ORF2

    Plasmid

    #200388

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MRAP_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176937

    Purpose

    Mammalian expression vector encoding MRAP and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MRAP (MRAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmCherry C1 MFF

    Plasmid

    #157760

    Purpose

    Expression of mcherry:MFF in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFF (MFF Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV2-miniSOG-VAMP2-T2A-mCherry

    Plasmid

    #50970

    Purpose

    AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    miniSOG-VAMP2-T2A-mCherry (Vamp2 Arabdopsis thaliana, Mouse)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

    Plasmid

    #132683

    Purpose

    AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Carnosine dipeptidase 1 (Cndp1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PVRL2-bio-His

    Plasmid

    #51824

    Purpose

    Expresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PVRL2 (NECTIN2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEX-SP-CFP-STIM1(23-685)

    Plasmid

    #18858

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted between residues 1-23 (signal peptid…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTAMAHISTEV_PfeAQ482A

    Plasmid

    #128945

    Purpose

    expresses Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV_-CMV-Pcdh15_mini_V4-CD2

    Plasmid

    #199184

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RRP1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176957

    Purpose

    Mammalian expression vector encoding RRP1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    RRP1 (RRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGP-CMV-GCaMP3-373

    Plasmid

    #91779

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP3-373
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-SENP6 (catalytic domain)

    Plasmid

    #16359

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP6 catalytic domain (SENP6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 628 to 1112 catalytic domain of human …

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAT15547_PEmax-SuperFi-Cas9

    Plasmid

    #184377

    Purpose

    Mammalian expression of SuperFi-Cas9 PEmax prime editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    PEmax-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS and c-myc NLS

    Expression

    Mammalian

    Mutation

    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-sh-Nix

    Plasmid

    #100770

    Purpose

    Lentiviral expression of shRNA targeting Nix

    Depositor

    Joseph Gordon

    Article

    da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

    Insert

    Lenti-sh-Nix
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GW1-mito-roGFP1-mApple

    Plasmid

    #107218

    Purpose

    Mammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    roGFP1-mApple
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC18T-mini-Tn7T-Gm-Pc-mKate2

    Plasmid

    #199249

    Purpose

    Fluorescent labelling of bacterial cells with mKate2

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized mKate2 gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid

    #171137

    Purpose

    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 221 to alanine, tryptophan 453…

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pClneoMyc human MST2

    Plasmid

    #37022

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

33. EGFP-Orf9b L52D

    Plasmid

    #165129

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf9b L52D
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HTRA1_PDZ_1

    Plasmid

    #103912

    Purpose

    Protein expression and purification of HTRA1 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    HTRA1 (HTRA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HDM_omicron_B11529_IDTDNA

    Plasmid

    #204142

    Purpose

    Expression plasmid for SARS-CoV-2 BA.1 variant spike

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. YFP-mDia2

    Plasmid

    #25420

    Depositor

    Arthur Alberts

    Article

    Wallar et al J Biol Chem. 2006 Feb 17. 281(7):4300-7.

    Insert

    diap3 (Diaph3 Mouse)
    

    Use

    Tags

    YFP A206K

    Expression

    Mammalian

    Mutation

    Encodes mDia2 amino acids T47 to L1171

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pTXB1-hMeCP2

    Plasmid

    #48091

    Purpose

    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mxe intein/chitin binding domain tagged

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

39. DLG4_PDZ_3

    Plasmid

    #103953

    Purpose

    Protein expression and purification of PSD95 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG4 (DLG4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GW1-mito-mCherry-roGFP2

    Plasmid

    #107220

    Purpose

    Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    mCherry-roGFP2
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-Ef1a-DIO-VGAT-WPRE-pA

    Plasmid

    #39320

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

    Plasmid

    #146392

    Purpose

    Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmeIF4G-trunc (eIF4G1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    322-1666 truncated version of elF4G sequence with…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. ePB-PURO-FLAG-METTL3

    Plasmid

    #160252

    Purpose

    For stable integration of FLAG-METTL3 in human cells with trasposase

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. GW1-mito-mRuby2-roGFP1

    Plasmid

    #107219

    Purpose

    Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

    Insert

    mRuby2-roGFP1
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCDNA3 T7 DLC1

    Plasmid

    #24266

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    DLC1 (DYNLL1, DLC1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

47. pcDNA3.1(+)mGAT1YFP8

    Plasmid

    #41669

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 8 C-terminal-m…

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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48. Igfbp1-promoter/pGL3

    Plasmid

    #12146

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    IGFBP1 promoter (Igfbp1 Rat)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains the canonical insulin response sequence …

    Promoter

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

49. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. MERS-coV-NSP3-c001

    Plasmid

    #173085

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    D1109-D1275, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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