We narrowed to 2,115 results for: D10

1. pCMV-mA3-BE3

   Plasmid

   #113419

   Purpose

   Expresses mA3-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hA3A-BE3-D131Y

   Plasmid

   #113424

   Purpose

   Expresses hA3A-BE3-D131Y in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-D131Y (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_D131Y

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTT3 Unc5B-FLAG

   Plasmid

   #72195

   Purpose

   Expresses full-length Unc5b with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5b (Unc5b Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9::BFP-humanized

   Plasmid

   #44247

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 fused to BFP
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS & tagBFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-LAMP1-mCherry-iLID

   Plasmid

   #174625

   Purpose

   Optogenetic coupling to lysosomes (LAMP1) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   LAMP1-mCherry-iLID (LAMP1 Synthetic, Human)
   

   Use

   Tags

   mCherry-iLID

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del

   Promoter

   Chicken beta-actin

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. hu-TLR4 cDNA wt pDEST40

   Plasmid

   #42646

   Purpose

   destination vector for expression of human TLR4 in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 (TLR4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. LLP339 pGK-dCas9-SunTag

    Plasmid

    #100943

    Purpose

    dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP5380 LAMP1-SBP- mCherry-FIREtag

    Plasmid

    #216725

    Purpose

    Expresses LAMP1-mCherry-FIREtag in mamallian cells

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    LAMP-1 (LAMP1 Human)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Delta(Pro-MP)ADAM10-HA

    Plasmid

    #65107

    Purpose

    Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 19-455

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNAZeo(-) LAMP2C

    Plasmid

    #89342

    Purpose

    Expresses human LAMP-2C in mammalian cells

    Depositor

    Janice Blum

    Article

    Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

    Insert

    Lysosomal-associated membrane protein 2C (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSI-Target-AID-NG

    Plasmid

    #119861

    Purpose

    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ICAM2-bio-His

    Plasmid

    #51758

    Purpose

    Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ICAM2 (ICAM2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP/SECFP-hATG16A1

    Plasmid

    #58994

    Purpose

    Expresses SECFP-hATG16A1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. LAMP1-mCherry-FRB*

    Plasmid

    #207145

    Purpose

    Lysosome-targeted rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    FRB* (T2098L mutant of FKBP-rapamycin binding dom…

    Expression

    Mammalian

    Mutation

    Silent mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Po (phoenol-inducible promoter)

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEX-SP-YFP-STIM1(23-685)

    Plasmid

    #18857

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP is inserted between residues 1-23 (signal pep…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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