We narrowed to 1,873 results for: FRT

1. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

   Plasmid

   #87827

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL652

   Plasmid

   #159456

   Purpose

   Donor empty vector with puromycin resistance gene expression cassette flanked by FRT

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAX6 CKO 2/7 Donor plasmid

   Plasmid

   #68467

   Purpose

   donor plasmid for targeting PAX6 locus

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   FRT flanked exon4 of PAX6 gene (PAX6 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK promoter

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

   Plasmid

   #90014

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-TNRC6C
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-eYFP-FKBP-MAD1-AA

   Plasmid

   #114038

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Use

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   MAD2 binding deficient MAD1 K541A/L543A

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-Adrm1-Flag

   Plasmid

   #19418

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

   Insert

   adhesion regulating molecule 1 (ADRM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

   Plasmid

   #90012

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Cdc25C
   NBirA*-GFP
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-FLJ20309

   Plasmid

   #15360

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   FLJ20309 106-544residues (INO80D Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. BirA-Myc_N_HNF1a

   Plasmid

   #167710

   Purpose

   Expresses HNF1a in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   HNF1a (HNF1A Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM296

    Plasmid

    #134180

    Purpose

    Mammalian expression of CENP-C and KNL2 CDK SA

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 HA-CCDC95

    Plasmid

    #19795

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    CCDC95 (INO80E Human)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

12. sgFFL_3x_siRNA

    Plasmid

    #59893

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--has three siRNA-like targets (fully c…

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

    Plasmid

    #215910

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332D, S1346D, S1616D, S169…

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

    Plasmid

    #215911

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A, S1616A, S169…

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5'-3xFLAG Mta1+exon minimal CMV pCDNA5

    Plasmid

    #212070

    Purpose

    LR vector for integration of Mta1+exon(12nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Mta1+exon(12nt) (Mta1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5'-3xFLAG Mta1-exon minimal CMV pCDNA5

    Plasmid

    #212071

    Purpose

    LR vector for integration of Mta1-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Mta1+exon(12nt) (Mta1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

    Plasmid

    #212078

    Purpose

    LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Zmynd8-exon (Zmynd8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5'-3xFLAG Sap30bp-exon minimal CMV pCDNA5

    Plasmid

    #212084

    Purpose

    LR vector for integration of Sap30bp-exon_siResist into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Sap30bp-exon_siResist (Sap30bp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TCTAACCATCTGCAGGACA

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5'-3xFLAG Sap30bp+exon minimal CMV pCDNA5

    Plasmid

    #212073

    Purpose

    LR vector for integration of Sap30bp+exon(48nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Sap30bp+exon(48nt) (Sap30bp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 5'-3xFLAG Smarcc2-exon minimal CMV pCDNA5

    Plasmid

    #212074

    Purpose

    LR vector for integration of Smarcc2-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Smarcc2-exon (Smarcc2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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