We narrowed to 9,811 results for: Met;
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Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS
Plasmid#140011PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sirius-mT-Sapphire_pcDNA3
Plasmid#89584PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertSirius-mT-Sapphire
ExpressionMammalianPromoterCMVAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGPI-SceI
Plasmid#32060PurposeConstruction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of BurkholderiaDepositorTypeEmpty backboneUseMutagenesis plasmidAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-mNeonGreen
Plasmid#162034PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsNeon GreenAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC53.2/PRMT5
Plasmid#35692DepositorInsertProtein arginine methyltransferase 5
PromoterSp6 for sense, T7 for sense and antisenseAvailable SinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAraH6HATT
Plasmid#63901PurposeFor expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.DepositorInsertTT Fab
Tags6xHis and HA tagExpressionBacterialPromoteraraBADAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
TagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaYin1 NES
Plasmid#33318DepositorInsertCaYin1 NES
TagsNES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY012 (pcDNA3.1-hCMtCpf1)
Plasmid#69984PurposeExpresses humanized CMtCpf1DepositorInserthCMtCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS
Plasmid#140008PurposePlasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsert4xPylT (Mma) cassette
ExpressionMammalianMutationU25CPromoterh7SKAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE30-mBFP
Plasmid#112682PurposeExpression of NADPH-dependent blue fluorescent proteinDepositorInsertHis-mBFP
TagsHis tagExpressionBacterialPromoterT5Available SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 CD mutant
Plasmid#89896PurposeExpresses mouse TET2 CD mutant in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
TagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHM_YEAST
Plasmid#204231PurposeBacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM_YEAST (MDH1 Budding Yeast)
Tags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A,H102A
Plasmid#159168PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A,H102A
TagsSV40 NLSExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits