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Flag-hPIASx alpha

Plasmid

#15209

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx alpha (PIAS2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-HBXIP

Plasmid

#42326

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2

Plasmid

#87034

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Use

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4A-pCMV7.1

Plasmid

#155022

Purpose

N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4A (CUL4A Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk3a1

Plasmid

#13758

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1 (MAPK10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse ULK2 (Ulk2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pCMV_NFL(K363TAG)-FLAG

Plasmid

#182656

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K363TAG

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag PRAS40

Plasmid

#14950

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Hu-Trip6

Plasmid

#27255

Depositor

Thomas Gilmore

Article

Zhao et al Gene Expr. 1999 . 8(4):207-17.

Insert

Trip6 (TRIP6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K5-9R

Plasmid

#63562

Purpose

Expression of human H2A with K5R and K9R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 5 and Lysine 9 are now Arginine

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG LIP

Plasmid

#15737

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC2

Plasmid

#173468

Purpose

Human PGRMC2 containing an N-terminal FLAG tag

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-GRM3-VN

Plasmid

#98965

Purpose

FLAG-tagged human mGluR3 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Use

Tags

FLAG (dykddddk) epitope tag, Signal/leader sequen…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luc-N

Plasmid

#58790

Purpose

Express LucN alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 1-398

Use

Tags

FLAG

Expression

Mammalian

Mutation

T576C,T591C to destroy MfeI and EcoRI sites

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luc-C

Plasmid

#58791

Purpose

Express LucC alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 394-550

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcFLAG RUNX3 (R122C)

Plasmid

#203438

Purpose

Mammalian expression of RUNX3

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge