We narrowed to 10,315 results for: plasmids 101

1. pQFD

   Plasmid

   #48101

   Purpose

   Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   PQ5

   Available Since

   Jan. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. dg263_pENTR_ChrimsonCel

   Plasmid

   #66101

   Purpose

   Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

   Depositor

   Dominique Glauser

   Article

   Schild et al Genetics. 2015 May 28. pii: genetics.115

   Insert

   Chrimson
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDL17

   Plasmid

   #106483

   Purpose

   pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   exo
   lacI
   rhaR
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. dSpCas9-VPR

   Plasmid

   #205247

   Purpose

   In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p4B5-EGFP

   Plasmid

   #140324

   Purpose

   Detection of Dengue virus infection in live cells

   Depositor

   Alan Rothman

   Article

   Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

   Insert

   DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   2 conservative n.t. differences near 5' end …

   Promoter

   CMV

   Available Since

   May 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. dSaCas9-VPR

   Plasmid

   #205251

   Purpose

   In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSaCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGDP1 NDM-1

   Plasmid

   #112883

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   NDM-1 (blaNDM-1 Klebsiella pneumoniae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGDP3 aph(3'')-Ia

   Plasmid

   #112879

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

    Plasmid

    #169533

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV PIG Pu.1

    Plasmid

    #66982

    Purpose

    Retroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Spi1 (Spi1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV Ef1a DIO GeNL_SS hGtACR1

    Plasmid

    #233511

    Purpose

    Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

    Inserts

    mNeonGreen
    ISN-IE
    hGtACR1
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Expression

    Mutation

    Promoter

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid

    #169490

    Purpose

    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-Basic-hCYP26A1P-E4-mCherry

    Plasmid

    #135478

    Purpose

    Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    mCherry Open reading Frame
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Short from promoter of human CYP26A1 (312 bp)

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

    Plasmid

    #169531

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-mCherry.3xFLAG-WPRE

    Plasmid

    #127861

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    mCherry
    

    Use

    AAV

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

    Plasmid

    #132682

    Purpose

    AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    AAV

    Tags

    6xHIS and Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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