We narrowed to 1,947 results for: 974

1. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

   Plasmid

   #222287

   Purpose

   Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   HOOK3 (HOOK3 Human)
   

   Use

   Tags

   8 His and ZZ tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MZB035

   Plasmid

   #217834

   Purpose

   Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Inserts

   AP3D1 (AP3D1 Human)
   AP3S1 (AP3S1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Removed residues 618-1153 from AP3D1

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TadA-N46L-NG-CBE

   Plasmid

   #227419

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   TadA-N46L-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. YEE-NG-CBE

   Plasmid

   #227418

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSD090A

   Plasmid

   #218132

   Purpose

   Hybrid circuit displaying mmCRBN bait and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   Crbn (Crbn Mouse)
   luxAB
   

   Use

   Tags

   434cI_RR69

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSD094B

   Plasmid

   #218131

   Purpose

   Hybrid circuit displaying mmCRBN bait and driving expression of gIII

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   Crbn (Crbn Mouse)
   M13 pIII
   

   Use

   Tags

   434cI_RR69

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

   Plasmid

   #212113

   Purpose

   Expression of GABARAP split tandem protein with mCherry

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mCherry-GABARAP (GABARAP Human, Discosoma sp.)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   G116A (lipidation deficiency) of upstream GABARAP

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

   Plasmid

   #212109

   Purpose

   Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR114-DuET

   Plasmid

   #213258

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR114 (ADGRG5 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGADT7-δ

    Plasmid

    #197259

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-3 δ (AP3D1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Val 549 Ile substitution; silent substitutions in…

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGADT7-ε

    Plasmid

    #197261

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-4 ε (AP4E1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitutions in codons 905, 1129 and 1137

    Promoter

    ADH1

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-ChBtW-mADAR2-short

    Plasmid

    #175588

    Purpose

    mammalian expression of mouse short Adar2, blasticidine selection

    Depositor

    Emanuela Felley-Bosco

    Article

    Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

    Insert

    Short Mouse Adar2
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CLDN8_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176963

    Purpose

    Mammalian expression vector encoding CLDN8 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CLDN8 (CLDN8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMKO.1-shCenpf

    Plasmid

    #160956

    Purpose

    Cenpf shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Cenpf shRNA (Cenpf Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. YCplac33-NAT_MCART1K91A

    Plasmid

    #140592

    Purpose

    Express MCART1K91A in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon-optimized for expression in S. cerevisiae; …

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

    Plasmid

    #105028

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

    Plasmid

    #105029

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 289aa Met133, 138, 186Ileu ELL2

    Plasmid

    #127272

    Purpose

    Expresses 289aa human ELL2 with Met133, 138, 186Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met133, 138, 186 Ileu, cut with XbaI blunt fillin

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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