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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 16,820 results for: URE

Showing: 2341 - 2370 of 16820 results

  1. GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

    Plasmid
    #166991
    Purpose
    Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(4), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    166991-plasmid-map-sequence-id-330472

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  2. GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

    Plasmid
    #166990
    Purpose
    Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(1), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    166990-plasmid-map-sequence-id-330470

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  3. GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

    Plasmid
    #166987
    Purpose
    Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
    Depositor
    Julius Lucks
    Article
    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.
    Insert
    Termination module:PolyU(4), Hairpin = 0 nt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    166987-plasmid-map-sequence-id-329691

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  5. pSTAP-seq_fly-4xUAS

    Plasmid
    #125149
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125149-plasmid-map-sequence-id-247994

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  10. pAB051e

    Plasmid
    #79195
    Purpose
    Directed Evolution of Protein-Protein Interactions
    Depositor
    David Liu
    Article
    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:
    Insert
    PExt-10cons lambda op-62 gIII, luxAB; Ptet lambda cI-SH2ABL1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PExt-10cons lambda op-62; Ptet
    Available since
    Availability
    Academic Institutions and Nonprofits only
    79195-plasmid-map-sequence-id-148316

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  13. pCMV-15F11-HA-Halo

    Plasmid
    #129592
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129592-plasmid-map-sequence-id-254268

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  15. pMitoTimer

    Plasmid
    #52659
    Purpose
    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo
    Depositor
    Zhen Yan
    Article
    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1
    Insert
    DsRed1-E5
    Use
    Dsred
    Tags
    human cytochrome c oxidase subunit VIII mitochond…
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    52659-plasmid-map-sequence-id-430007

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  18. pX333

    Plasmid
    #64073
    Purpose
    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    3XFLAG-Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CBh; U6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64073-plasmid-map-sequence-id-360030

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  21. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  24. pCMV-15F11-HA-mEGFP

    Plasmid
    #129590
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129590-plasmid-map-sequence-id-254270

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  27. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    U6 and CBh
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2341 - 2370 of 16820 results