We narrowed to 18,106 results for: URE

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pMIIS928

Plasmid

#238211

Purpose

Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

HA-NLS

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMW363

Plasmid

#238208

Purpose

Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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mCh-Sec61 beta

Plasmid

#49155

Purpose

expression of Sec61 beta fused to mCherry, used as a general ER marker

Depositor

Gia Voeltz

Article

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Tags

mCherry*

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2013

Availability

Academic Institutions and Nonprofits only

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BFP-Sec61 beta

Plasmid

#49154

Purpose

expression of Sec61 beta fused to TagBFP, used as a general ER marker

Depositor

Gia Voeltz

Article

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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piggyFlex

Plasmid

#218234

Purpose

A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

Depositor

Jay Shendure

Article

Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAB088c

Plasmid

#79213

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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FH-TET3-pEF

Plasmid

#49446

Purpose

This plasmid expresses full-length human TET3.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

Tet methylcytosine dioxygenase 3 (TET3 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd

Plasmid

#80950

Purpose

Expresses Gsdmd in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Prolactin-CD4d3+4-bio

Plasmid

#73123

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Prolactin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PRL (PRL Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-LK03

Plasmid

#206512

Purpose

AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

Insert

LK03 capsid

Use

AAV

Promoter

p5

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-GFP

Plasmid

#106281

Purpose

A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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LPC-flag-REST-WT

Plasmid

#41903

Depositor

Stephen Elledge

Article

Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

Insert

RE1-silencing transcription factor (REST Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Article

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

LTR7

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pENV8(GAAA)-GFPuv

Plasmid

#70128

Purpose

Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

Depositor

Robert Batey

Article

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pT7C-BAG2

Plasmid

#177654

Purpose

Recombinant protein synthesis of human BAG2

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

BAG2 (BAG2 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(+)-Fc

Plasmid

#59314

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFW2500

Plasmid

#224213

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1 and recT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM4Di-mCherry

Plasmid

#161579

Purpose

To express hM4Di in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF hM3Dq-mCherry

Plasmid

#161578

Purpose

To express hM3Dq in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM3Dq-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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C8orf55-CD4d3+4-bio

Plasmid

#73126

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C8orf55 (THEM6 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO MYC3

Plasmid

#177848

Purpose

Bacterial Expression of MYC3

Depositor

Karsten Melcher

Article

Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

Insert

MYC3 (MYC3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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FH-TET3-HxDmut-pEF

Plasmid

#127896

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-HxDmut (TET3 Human)

Tags

HA and Flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn–GFP

Plasmid

#35958

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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