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pBa.GFP-KIF3B tail 391-747

Plasmid

#134615

Purpose

Mammalian expression of mouse KIF3B 391-747

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 390 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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shFGF2#2

Plasmid

#157668

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-GS-KIF3A tail 419-702

Plasmid

#134614

Purpose

Mammalian expression of human KIF3A 419-702

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3A (KIF3A Human)

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT with deletion of the 418 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP2.GFP

Plasmid

#109140

Purpose

GFP-tagged CYFIP2 expression construct

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-mATG5

Plasmid

#58739

Purpose

Expresses Venus-mATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

Venus

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-SFPQY527A

Plasmid

#166961

Purpose

Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Mutation

SFPQ-Y527A

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-iLID-mCherry-RAB11

Plasmid

#174623

Purpose

Optogenetic coupling to recycling endosomes (RAB11) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

iLID-mCherry-RAB11 (RAB11A Human, Synthetic)

Tags

iLID-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only