Addgene

Showing: 141 - 160 of 12487 results

Results per page:

Sort results by:

pFBOH-LIC_DDA1:1-102

Plasmid

#210902

Purpose

Insect expression of full-length human DDA1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDA1:M1-T102 (DDA1 Human)

Use

Tags

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFBOH-LIC_DDB1:1-1140

Plasmid

#210886

Purpose

Insect expression of full-length human DDB1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140 (DDB1 Human)

Use

Tags

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFBOH-LIC_GRWD1:1-446

Plasmid

#210876

Purpose

Insect expression of full-length human GRWD1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

GRWD1:M1-V446 (GRWD1 Human)

Use

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLIC-GST-GINIP

Plasmid

#204844

Purpose

Express GST tagged-GINIP in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

PHF24 (KIAA1045 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab2b

Plasmid

#203692

Purpose

Expresses N8C_Fab2b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab2b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab5a

Plasmid

#203693

Purpose

Expresses N8C_Fab5a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab5a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab2a

Plasmid

#203688

Purpose

Expresses N8C_Fab2a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab2a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab1b

Plasmid

#203691

Purpose

Expresses N8C_Fab1b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab1b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1+HIF1a-AS1_1200_unspliced

Plasmid

#194176

Purpose

Expresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIC-His-GBAi

Plasmid

#171755

Purpose

For the expression of His-GBAi in bacteria

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEU3-NII-HxHLICNot

Plasmid

#140183

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

12xHis

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSlick-Neo-KANK1

Plasmid

#121983

Purpose

Tetracycline-inducible expression of untagged full-length KANK1

Depositor

GuangJun Zhang

Article

Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

Insert

KANK1 (KANK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Tight TRE promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 LIC cloning vector (6A)

Plasmid

#30124

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-FLAG-Dicer-delHelicC

Plasmid

#51366

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to HelicC domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS005: superecliptic pHluorin (cytosolic)

Plasmid

#163049

Purpose

superecliptic pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Use

Tags

TEV-GFP-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

Plasmid

#111903

Purpose

Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

Depositor

Andrew Carter

Inserts

His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
DYNC1I2 (DYNC1I2 Human)
DYNC1LI2 (DYNC1LI2 Human)
DYNLT1 (DYNLT1 Human)
DYNLRB1 (DYNLRB1 Human)
DYNLL1 (DYNLL1 Human)

Use

Tags

8xHis, SNAPf-tag, TEV site, and ZZ-tag

Expression

Insect

Mutation

Optimised for expression in Sf9 cells.

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET LIC cloning vector (2A-T)

Plasmid

#29665

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG

Plasmid

#169948

Purpose

Mammalian expression of CARD8-FLAG

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge